Sensitive detection of point mutation using exponential strand displacement amplification-based surface enhanced Raman spectroscopy

• Published in: Biosensors & bioelectronics Vol. 65; pp. 191 - 197
• Main Authors: Huang, Si-qiang, Hu, Juan, Zhu, Guichi, Zhang, Chun-yang
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.03.2015
• Subjects: Amplification; Biosensing Techniques - methods; Biosensors; Diagnosis; Displacement; Exponential strand displacement amplification; Genes, ras; HEK293 Cells; Humans; K-ras gene; Mutations; Nucleic Acid Amplification Techniques - methods; Point Mutation; Raman spectroscopy; Recognition; Spectrum Analysis, Raman - methods; Strands; Surface enhanced Raman spectroscopy; Exponential strand displacement amplification; K-ras gene; Point mutation; Surface enhanced Raman spectroscopy

Accurate identification of point mutation is particularly imperative in the field of biomedical research and clinical diagnosis. Here, we develop a sensitive and specific method for point mutation assay using exponential strand displacement amplification (SDA)-based surface enhanced Raman spectrosco...