In-depth Site-specific Analysis of N-glycoproteome in Human Cerebrospinal Fluid and Glycosylation Landscape Changes in Alzheimer's Disease

• Published in: Molecular & cellular proteomics Vol. 20; p. 100081
• Main Authors: Chen, Zhengwei, Yu, Qinying, Yu, Qing, Johnson, Jillian, Shipman, Richard, Zhong, Xiaofang, Huang, Junfeng, Asthana, Sanjay, Carlsson, Cynthia, Okonkwo, Ozioma, Li, Lingjun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2021; American Society for Biochemistry and Molecular Biology
• Subjects: Alzheimer Disease - cerebrospinal fluid; Cell Line; cerebrospinal fluid; electron-transfer higher-energy collision induced dissociation (EThcD); glycopeptide enrichment; Glycopeptides - cerebrospinal fluid; Glycoproteins - cerebrospinal fluid; Glycosylation; Humans; N-glycoproteome analysis; Proteome - analysis; site-specific intact glycopeptide characterization; APP; ABC; site-specific intact glycopeptide characterization; AD; SDS; DMSO; DTT; IAA; PTM; glycopeptide enrichment; CNS; ADRC; EThcD; PBA; TFA; N-glycoproteome analysis; WGA; RCA; electron-transfer higher-energy collision induced dissociation (EThcD); FDR; cerebrospinal fluid; CSF; HILIC; ConA

As the body fluid that directly interchanges with the extracellular fluid of the central nervous system (CNS), cerebrospinal fluid (CSF) serves as a rich source for CNS-related disease biomarker discovery. Extensive proteome profiling has been conducted for CSF, but studies aimed at unraveling site-...