Indole Channeling by Tryptophan Synthase of Neurospora

• Published in: The Journal of biological chemistry Vol. 249; no. 13; pp. 4041 - 4049
• Main Author: Matchett, William H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.07.1974; American Society for Biochemistry and Molecular Biology
• Subjects: Binding Sites; Biological Evolution; Carbon Radioisotopes; Glycerophosphates - metabolism; Indoles - metabolism; Kinetics; Mathematics; Neurospora - enzymology; Neurospora crassa - enzymology; plant biochemistry; plant physiology; Pyridoxal Phosphate - pharmacology; Serine - pharmacology; Time Factors; Tryptophan - biosynthesis; Tryptophan Synthase - metabolism

Tryptophan synthase (l-serine hydro-lyase-adding indole) (EC 4.2.1.20) of Neurospora crassa was studied in reaction mixtures containing [2-14C]indoleglycerolphosphate and non-labeled indole. A detailed kinetic analysis of the passage of isotope from indoleglycerolphosphate to indole and to tryptopha...