A Simple Microplate Assay for Reactive Oxygen Species Generation and Rapid Cellular Protein Normalization

• Published in: Bio-protocol Vol. 11; no. 1; p. e3877
• Main Authors: Ng, Neville, Ooi, Lezanne
• Format: Journal Article
• Language: English
• Published: United States Bio-Protocol 05.01.2021; Bio-protocol LLC
• Subjects: Biology; Methods; Dichlorofluorescein; Oxidative stress; Reactive oxygen species; SRB; DCF; Normalization assay; Sulforhodamine

2',7'-dichlorofluorescein (DCF) and derivatives are commonly used as fluorescent indicators of a broad spectrum of reactive oxygen species (ROS) generation in cell-based assays. However, there are numerous challenges inherent to the utilization of DCF probes for intracellular microscopic a...