Enhanced Enzymatic Activity of Laccase (from Pycnoporus sanguineus CS43) Immobilized on Sputtered Nanostructured Gold Thin Films

• Published in: Journal of nanoscience and nanotechnology Vol. 17; no. 2; p. 939
• Main Authors: Rodríguez-Delgado, Melissa, Ornelas-Soto, Nancy, Martínez-Lorán, Erick, Hernandez-Luna, Carlos, García-García, Alejandra, Contreras-Torres, Flavio F
• Format: Journal Article
• Language: English
• Published: United States 01.02.2017
• Subjects: Biosensing Techniques; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Fatty Alcohols - chemistry; Fungal Proteins - chemistry; Fungal Proteins - metabolism; Gold - chemistry; Laccase - chemistry; Laccase - metabolism; Nanostructures - chemistry; Palmitic Acids - chemistry; Particle Size; Pycnoporus - enzymology; Sulfhydryl Compounds - chemistry; Surface Properties; Gold; Nanostructured Thin Films; Enzyme Immobilizaion; Laccase

Functionalization of thin films with organic ligands has been the subject of intense research due to their potential application as heterogeneous molecular nanosystems. In this work, self-assembled monolayers of thiols (16-mercaptohexadecanoic acid and 11-mercaptoundecanol) were used to bind laccase...