Single‐Cell Transcriptomics of Engineered Cardiac Tissues From Patient‐Specific Induced Pluripotent Stem Cell–Derived Cardiomyocytes Reveals Abnormal Developmental Trajectory and Intrinsic Contractile Defects in Hypoplastic Right Heart Syndrome

• Published in: Journal of the American Heart Association Vol. 9; no. 20; p. e016528
• Main Authors: Lam, Yin‐Yu, Keung, Wendy, Chan, Chun‐Ho, Geng, Lin, Wong, Nicodemus, Brenière‐Letuffe, David, Li, Ronald A., Cheung, Yiu‐Fai
• Format: Journal Article
• Language: English
• Published: England John Wiley and Sons Inc 20.10.2020; Wiley
• Subjects: Bioengineering; Heart Defects, Congenital - genetics; Heart Defects, Congenital - pathology; Heart Defects, Congenital - physiopathology; Humans; hypoplastic right heart syndrome; induced pluripotent stem cells; Induced Pluripotent Stem Cells - physiology; Models, Cardiovascular; Models, Genetic; Myocardial Contraction; Myocytes, Cardiac - physiology; Organ Culture Techniques; Original Research; Pulmonary Atresia - genetics; Pulmonary Atresia - pathology; Pulmonary Atresia - physiopathology; pulmonary atresia with intact ventricular septum; single cell transcriptomics; Tissue Engineering - methods; Transcriptome; hypoplastic right heart syndrome; induced pluripotent stem cells; single cell transcriptomics; pulmonary atresia with intact ventricular septum

Background To understand the intrinsic cardiac developmental and functional abnormalities in pulmonary atresia with intact ventricular septum (PAIVS) free from effects secondary to anatomic defects, we performed and compared single-cell transcriptomic and phenotypic analyses of patient- and healthy...