Post-Golgi anterograde transport requires GARP-dependent endosome-to-TGN retrograde transport

• Published in: Molecular biology of the cell Vol. 26; no. 17; pp. 3071 - 3084
• Main Authors: Hirata, Tetsuya, Fujita, Morihisa, Nakamura, Shota, Gotoh, Kazuyoshi, Motooka, Daisuke, Murakami, Yoshiko, Maeda, Yusuke, Kinoshita, Taroh
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 01.09.2015
• Subjects: Biological Transport; Cell Culture Techniques; Endosomes - metabolism; Gene Knockout Techniques; Glycosylphosphatidylinositols - metabolism; Golgi Apparatus - metabolism; GPI-Linked Proteins - metabolism; HEK293 Cells; HeLa Cells; Humans; Membrane Proteins - metabolism; Protein Transport; RNA Interference; SNARE Proteins - metabolism; trans-Golgi Network - metabolism; Vesicular Transport Proteins - genetics; Vesicular Transport Proteins - metabolism

The importance of endosome-to– trans-Golgi network (TGN) retrograde transport in the anterograde transport of proteins is unclear. In this study, genome-wide screening of the factors necessary for efficient anterograde protein transport in human haploid cells identified subunits of the Golgi-associa...