Histidine placement in de novo–designed heme proteins

• Published in: Protein science Vol. 8; no. 9; pp. 1888 - 1898
• Main Authors: GIBNEY, BRIAN R., DUTTON, P. LESLIE
• Format: Journal Article
• Language: English
• Published: Bristol Cambridge University Press 01.09.1999; Cold Spring Harbor Laboratory Press
• Subjects: Amino Acid Sequence; Circular Dichroism; de novo design; electrochemistry; Electron Spin Resonance Spectroscopy; Ferric Compounds - chemistry; Ferric Compounds - metabolism; heme protein; Hemeproteins - chemical synthesis; Hemeproteins - metabolism; Histidine - metabolism; maquette; metalloprotein; Metalloproteins - chemical synthesis; Metalloproteins - metabolism; Molecular Sequence Data; Oxidation-Reduction; Peptides - chemistry; Peptides - metabolism; Protein Denaturation; Protein Engineering; Protein Structure, Secondary; Thermodynamics; maquette; de novo design; metalloprotein; electrochemistry; heme protein

The effects of histidine residue placement in a de novo-designed four-α-helix bundle are investigated by placement of histidine residues at coiled coil heptad a positions in two distinct heptads and at each position within a single heptad repeat of our prototype heme protein maquette, [H10H24]2 [{Ac...