DNA probe and PCR‐specific reaction for Lactobacillus plantarum

• Published in: Journal of applied microbiology Vol. 82; no. 6; pp. 783 - 790
• Main Authors: Quere, F., Deschamps, A., Urdaci, M. C.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.06.1997; Blackwell Science
• Subjects: Bacteriology; Base Sequence; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; DNA Probes; DNA, Bacterial - analysis; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Genetics; Lactobacillus - genetics; Lactobacillus - isolation & purification; Lactobacillus plantarum; Microbiology; Mission oriented research; Molecular Sequence Data; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Polymerase chain reaction; Molecular hybridization; Lactobacillus plantarum; Nucleotide sequence; Primer; Bacteria; Lactobacillaceae; Identification; Molecular probe; Molecular cloning; Detection; Sequencing

A 300 bp DNA fragment of Lactobacillus plantarum isolated by randomly amplified polymorphic DNA (RAPD) analysis was cloned and sequenced. This fragment was tested using a dot‐blot DNA hybridization technique for its ability to identify Lact. plantarum strains. This probe hybridized with all Lact. pl...