DNA probe and PCR‐specific reaction for Lactobacillus plantarum

• Published in: Journal of applied microbiology Vol. 82; no. 6; pp. 783 - 790
• Main Authors: Quere, F., Deschamps, A., Urdaci, M. C.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.06.1997; Blackwell Science
• Subjects: Bacteriology; Base Sequence; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; DNA Probes; DNA, Bacterial - analysis; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Genetics; Lactobacillus - genetics; Lactobacillus - isolation & purification; Lactobacillus plantarum; Microbiology; Mission oriented research; Molecular Sequence Data; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Polymerase chain reaction; Molecular hybridization; Lactobacillus plantarum; Nucleotide sequence; Primer; Bacteria; Lactobacillaceae; Identification; Molecular probe; Molecular cloning; Detection; Sequencing
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1046/j.1365-2672.1997.00157.x

A 300 bp DNA fragment of Lactobacillus plantarum isolated by randomly amplified polymorphic DNA (RAPD) analysis was cloned and sequenced. This fragment was tested using a dot‐blot DNA hybridization technique for its ability to identify Lact. plantarum strains. This probe hybridized with all Lact. plantarum strains tested and with some strains of Lact. pentosus,albeit more weakly. Two internal primers of this probe were selected (LbPl1 and LbPl2) and polymerase chain reaction (PCR) was carried out. All Lact. plantarum strains tested amplified a 250 bp fragment contrary to the other LAB species tested. This specific PCR for Lact. plantarum was also performed from colonies grown on MRS medium with similar results. These methods enabled the rapid and specific detection and identification of Lact. plantarum.