Enhanced interferon regulatory factor 3 binding to the interleukin-23p19 promoter correlates with enhanced interleukin-23 expression in systemic lupus erythematosus

• Published in: Arthritis & rheumatology (Hoboken, N.J.) Vol. 64; no. 5; pp. 1601 - 1609
• Main Authors: Smith, Siobhán, Gabhann, Joan Nı´, Higgs, Rowan, Stacey, Kevin, Wahren-Herlenius, Marie, Espinosa, Alexander, Totaro, Maria Grazia, Sica, Antonio, Ball, Elizabeth, Bell, Aubrey, Johnston, James, Browne, Peter, O'Neill, Lorraine, Kearns, Grainne, Jefferies, Caroline A.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2012; Wiley; Wiley Subscription Services, Inc
• Subjects: Animals; Biological and medical sciences; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Chromatin Immunoprecipitation; Disease Models, Animal; Diseases of the osteoarticular system; Female; Gene Expression Regulation; Genes; Humans; Interferon Regulatory Factor-3 - genetics; Interferon Regulatory Factor-3 - metabolism; Interleukin-23 Subunit p19 - genetics; Interleukin-23 Subunit p19 - metabolism; Kinases; Lupus; Lupus Erythematosus, Systemic - metabolism; Macrophages - drug effects; Macrophages - metabolism; Male; Medical sciences; Mice; Mice, Inbred C57BL; Mice, Knockout; Monocytes - metabolism; Poly I-C - pharmacology; Protein Array Analysis - methods; Protein Binding; Protein-Serine-Threonine Kinases - pharmacology; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Toll-Like Receptor 3 - immunology; Promoter; Immunopathology; Connective tissue disease; Skin disease; Systemic lupus erythematosus; Systemic disease; Rheumatology; Autoimmune disease; Interferon

Objective To examine the role of interferon regulatory factor 3 (IRF‐3) in the regulation of interleukin‐23 (IL‐23) production in patients with systemic lupus erythematosus (SLE). Methods Bone marrow–derived macrophages were isolated from both wild‐type and IRF3−/− C57BL/6 mice. These cells were sti...