Proximity ligation assays for isoform-specific Akt activation in breast cancer identify activated Akt1 as a driver of progression

• Published in: The Journal of pathology Vol. 227; no. 4; pp. 481 - 489
• Main Authors: Spears, Melanie, Cunningham, Carrie A, Taylor, Karen J, Mallon, Elizabeth A, Thomas, Jeremy St J, Kerr, Gillian R, Jack, Wilma JL, Kunkler, Ian H, Cameron, David A, Chetty, Udi, Bartlett, John MS
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.08.2012; Wiley
• Subjects: Akt; Biological and medical sciences; breast cancer; Breast Neoplasms - metabolism; Breast Neoplasms - physiopathology; Cohort Studies; Disease Progression; Female; Gynecology. Andrology. Obstetrics; Humans; Investigative techniques, diagnostic techniques (general aspects); Mammary gland diseases; Medical sciences; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Phosphorylation - physiology; Prognosis; Protein Isoforms - metabolism; Proto-Oncogene Proteins c-akt - metabolism; proximity ligation assay; Reproducibility of Results; Tumors; Molecular form; Breast disease; Proximity; Akt protein kinase; Activation; Breast cancer; Malignant tumor; Akt; proximity ligation assay; Assay; Mammary gland diseases; Anatomic pathology; Ligature; Cancer

The PI3K/Akt signal transduction pathway plays an important role in cancer progression and cell survival. Akt activation is associated with poor outcome in endocrine‐treated breast cancer, whereas high levels of cytoplasmic Akt2 are associated with an improved overall survival. Proximity ligation as...