Mutational analysis of exopolysaccharide biosynthesis by Lactobacillus sakei 0-1

• Published in: FEMS microbiology letters Vol. 169; no. 2; pp. 241 - 249
• Main Authors: Breedveld, M, Bonting, K, Dijkhuizen, L
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 15.12.1998; Blackwell; Oxford University Press
• Subjects: Bacterial Capsules - biosynthesis; Bacteriology; Biological and medical sciences; Biosynthesis; Cell morphology; Cell walls; Cytology; dTDP‐rhamnose; Ethyl Methanesulfonate; Exopolysaccharides; Extracellular polysaccharide; food microbiology; food processing; Fundamental and applied biological sciences. Psychology; Glucokinase - metabolism; Glucose - biosynthesis; Hydro-Lyases - metabolism; Lactic acid bacterium; Lactobacillus - drug effects; Lactobacillus - metabolism; Lactobacillus sakei; meat products; Metabolism. Enzymes; Microbiology; Microscopy, Electron; Morphology; Mutagens; Mutants; Mutation - drug effects; Non‐ropy mutant; Nucleotidyltransferases - metabolism; Phosphoglucomutase - metabolism; Polysaccharides, Bacterial - biosynthesis; Rhamnose; Rhamnose - biosynthesis; Sulfonic acid; Temperature dependence; UDP‐glucose; Extracellular polysaccharide; Lactic acid bacterium; dTDP-rhamnose; Non-ropy mutant; UDP-glucose; Lactic acid bacteria; Enzymatic activity; Morphology; Bacteria; Lactobacillaceae; Biosynthesis; Mutation; Metabolism; Extracellular; Lactobacillus sake; Polysaccharide; Cell wall

Lactobacillus sakei strain 0-1 produces an exopolysaccharide (EPS) consisting of glucose and rhamnose in a ratio of 3:2. As part of a biochemical and molecular analysis of the EPS biosynthetic pathway in L. sakei strain 0-1, we have isolated a random set of EPS-negative mutants. Following treatment...