Reduced postnatal cerebral glucose metabolism measured by PET after asphyxia in near term fetal lambs

• Published in: Journal of neuroscience research Vol. 66; no. 5; pp. 844 - 850
• Main Authors: Thorngren-Jerneck, Kristina, Ley, David, Hellström-Westas, Lena, Hernandez-Andrade, Edgar, Lingman, Göran, Ohlsson, Tomas, Oskarsson, Gylfi, Pesonen, Erkki, Sandell, Anders, Strand, Sven-Erik, Werner, Olof, Maršal, Karel
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01.12.2001
• Subjects: Acidosis - diagnostic imaging; Acidosis - etiology; Animals; Animals, Newborn - metabolism; asphyxia; Asphyxia Neonatorum - diagnostic imaging; Basic Medicine; Blood Pressure - physiology; Cerebral Cortex - diagnostic imaging; cerebral glucose metabolism; Cerebrovascular Circulation - physiology; Disease Models, Animal; Electroencephalography; Female; Fetal Hypoxia - diagnostic imaging; fetal lamb; Fetus - metabolism; Fetus - physiopathology; Glucose - metabolism; Heart Arrest - metabolism; Heart Arrest - physiopathology; Humans; Hypoxia-Ischemia, Brain - diagnostic imaging; Infant, Newborn; Lactic Acid - metabolism; Male; Medical and Health Sciences; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Nerve Degeneration - diagnostic imaging; Neurosciences; Neurovetenskaper; positron emission tomography; Pregnancy; Sheep; Tomography, Emission-Computed; Umbilical Cord - injuries
• ISSN: 0360-4012; 1097-4547; 1097-4547
• DOI: 10.1002/jnr.10051

The effects of fetal asphyxia on cerebral function and development, involve the transition from fetal to neonatal life. Changes in cerebral glucose metabolism may be an early postnatal indicator of fetal asphyxia. The objective is to develop an experimental lamb model involving the transition from fetal to neonatal life and to examine the effect of fetal asphyxia with cerebral hypoxic ischemia on early postnatal cerebral glucose metabolism. Fetal asphyxia was induced by total umbilical cord occlusion in eight near‐term fetal lambs (134–138 days) with the ewe under isoflurane‐opiate anesthesia. The mean occlusion time until cardiac arrest was 14.5 (4.2) min (SD). Lambs were immediately delivered and standardized resuscitation was instituted after 2 min asystole. At 4 hr postnatal age, [18‐F]Fluoro‐2‐deoxy‐glucose (18‐FDG) was injected intravenously in eight asphyxiated lambs and in eight controls. Cerebral glucose metabolism was examined by positron emission tomography (PET). As a result the mean arterial blood pressure, acid‐base values, blood glucose and serum lactate at 4 hr postnatal age did not differ significantly between lambs subjected to umbilical cord occlusion and controls. EEG was abnormal in all lambs subjected to cord occlusion and normal in the controls at 4 hr postnatal age. Global cerebral metabolic rate (CMRgl) as determined by PET was significantly lower in lambs subjected to cord occlusion mean/median (SD) 22.2/19.6 (8.4) μmol/min/100 g) than in controls mean/median (SD) 37.8/35.9 (6.1); P < 0.01). Global CMRgl is significantly reduced in newborn lambs 4 hr after fetal asphyxia induced by umbilical cord occlusion. A reduction in CMRgl is an early indicator of global hypoxic cerebral ischemia. © 2001 Wiley‐Liss, Inc.