Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches

• Published in: The Plant journal : for cell and molecular biology Vol. 110; no. 1; pp. 129 - 146
• Main Authors: Xu, Hui, Li, Zhen, Jiang, Peng‐Fei, Zhao, Li, Qu, Chang, Van de Peer, Yves, Liu, Yan‐Jing, Zeng, Qing‐Yin
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.04.2022
• Subjects: active site motif; active sites; Catalytic Domain; Divergence; Enzymatic activity; enzymatic specificity; Enzymes; evolution; functional divergence; Gene duplication; Gene expression; Gene Expression Regulation, Plant - genetics; Gene families; gene family; Genes; Genomes; Glutaredoxin; Glutaredoxins - genetics; Humans; Localization; Molecular evolution; Phylogeny; Plant Proteins - metabolism; Poplar; Populus; Populus - metabolism; Populus trichocarpa; Proteins; Site-directed mutagenesis; Substrate specificity; Substrates; Switches; functional divergence; Populus trichocarpa; enzymatic specificity; gene family; glutaredoxin; active site motif

SUMMARY Enzymes are essential components of all biological systems. The key characteristics of proteins functioning as enzymes are their substrate specificities and catalytic efficiencies. In plants, most genes encoding enzymes are members of large gene families. Within such families, the contributi...