Cloning and expression of pathogenesis-related protein 4 from jelly fig (Ficus awkeotsang Makino) achenes associated with ribonuclease, chitinase and anti-fungal activities

• Published in: Plant physiology and biochemistry Vol. 56; pp. 1 - 13
• Main Authors: Lu, Hsi-Chi, Lin, Jia-Hui, Chua, Anna C.N., Chung, Tse-Yu, Tsai, I.-Chun, Tzen, Jason T.C., Chou, Wing-Ming
• Format: Journal Article
• Language: English
• Published: Paris Elsevier Masson SAS 01.07.2012; Elsevier
• Subjects: Achene; achenes; Amino Acid Sequence; Anti-fungal; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; antifungal properties; Athelia rolfsii; Biological and medical sciences; Chitin - metabolism; Chitinase; Chitinases - metabolism; Cloning, Molecular; DNA, Complementary; Ficus; Ficus - chemistry; Ficus awkeotsang; Ficus pumila var. awkeotsang; Fundamental and applied biological sciences. Psychology; Fungi - drug effects; Fusarium - drug effects; Fusarium oxysporum; Gene Expression; heat treatment; Heat treatments; Hot Temperature; Komagataella pastoris; Molecular Sequence Data; Pathogenesis-related protein 4; Pathogenesis-related proteins; Pericarp; Pichia - metabolism; Pichia pastoris; plant physiology; Plant physiology and development; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Proteins - pharmacology; Polymerase chain reaction; Recombinants; Ribonuclease; ribonucleases; Ribonucleases - genetics; Ribonucleases - metabolism; Ribonucleases - pharmacology; RNA - metabolism; Sclerotium rolfsii; Seeds - chemistry; Thermal stability; yeasts; ChtBD; Pathogenesis-related protein 4; Anti-fungal; Achene; MALDI/MS; Pericarp; Ficus awkeotsang; Ribonuclease; PR-4; Chitinase; Enzyme; Esterases; Moraceae; Woody plant; Dry fruit; Pathogenesis related protein; Biological activity; Glycosylases; Antifungal agent; Plant physiology; Ficus; Dicotyledones; Glycosidases; Angiospermae; Hydrolases; Spermatophyta

A cDNA fragment (FaPR4) encoding a class I pathogenesis-related protein 4 (PR-4) from Ficus awkeotsang was obtained by PCR cloning. Plant PR-4s were grouped into class I and II, differing by the presence of ChtBD and hinge. The predicted mature FaPR4 comprises N-terminal chitin-binding domain (ChtBD...