Enhancing the biocatalytic potential of carbonyl reductase of Candida viswanathii using aqueous-organic solvent system

• Published in: Bioresource technology Vol. 100; no. 3; pp. 1041 - 1047
• Main Authors: Kansal, Himani, Banerjee, Uttam Chand
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.02.2009; Elsevier
• Subjects: Activity retention; Alcohol Oxidoreductases - metabolism; Biological and medical sciences; Biotechnology; Candida; Candida - classification; Candida - enzymology; Candida viswanathii; Catalysis; Cell Survival; Cells, Immobilized - drug effects; Enzyme Activation; Enzyme Stability; Fundamental and applied biological sciences. Psychology; Organic Chemicals - chemistry; Organic Chemicals - pharmacology; Oxido-reductase; Solvent cell system; Solvents - chemistry; Solvents - pharmacology; Substrate tolerance; Water - metabolism; Solvent cell system; Activity retention; Oxido-reductase; Substrate tolerance; Candida viswanathii; Enzyme; Tolerance; Carbonyl; Biocatalyst; Fungi; Substrate; Organic solvent; Aqueous medium; Fungi Imperfecti; Oxidoreductases; Reductase

In the present work, the toxic effect of various solvents with different Log P values was studied on the whole cells of Candida viswanathii. Experiments showed that the lower concentrations of some solvent increased both the activity retention and enzyme activity as compared to the control while thi...