State of the Art of Pathologic and Molecular Testing

• Published in: Hematology/oncology clinics of North America Vol. 37; no. 3; p. 463
• Main Author: Dacic, Sanja
• Format: Journal Article
• Language: English
• Published: United States 01.06.2023
• Subjects: Biomarkers, Tumor - genetics; Carcinoma, Non-Small-Cell Lung - diagnosis; Carcinoma, Non-Small-Cell Lung - genetics; High-Throughput Nucleotide Sequencing - methods; Humans; Lung Neoplasms - diagnosis; Lung Neoplasms - genetics; Molecular Diagnostic Techniques; Mutation; Tumor Microenvironment; PD-L1; Molecular testing; NGS-AI; Lung cancer
• DOI: 10.1016/j.hoc.2023.02.001

Advances in the treatment of non-small cell lung carcinoma have resulted in improved histologic classification and the implementation of molecular testing for predictive biomarkers into the routine diagnostic workflow. Over the past decade, molecular testing has evolved from single-gene assays to high-thoroughput comprehensive next-generation sequencing. Economic barriers, suboptimal turnaround time to obtain the results, and limited tissue available for molecular assays resulted in adoption of liquid biopsies (ctDNA) into clinical practice. Multiplex immunohistochemical/immunofluorescence assays evaluating tumor microenvironment together with the AI approaches are anticipated to translate from research into clinical care.