A novel RT-multiplex PCR for enteroviruses, hepatitis A and E viruses and influenza A virus among infants and children with diarrhea in Vietnam

• Published in: Archives of virology Vol. 150; no. 6; pp. 1175 - 1185
• Main Authors: Phan, T. G., Nguyen, T. A., Yan, H., Okitsu, S., Ushijima, H.
• Format: Journal Article
• Language: English
• Published: Wien Springer 01.06.2005; New York, NY Springer Nature B.V
• Subjects: Adenovirus; Biological and medical sciences; Child; Child, Preschool; Children; Diarrhea; Diarrhea - diagnosis; Diarrhea - virology; DNA Primers; Enterovirus - classification; Enterovirus - genetics; Enterovirus - isolation & purification; Enteroviruses; Epidemiology; Feces - virology; Fundamental and applied biological sciences. Psychology; Gastroenteritis; Hepatitis A; Hepatitis A virus; Hepatitis A Virus, Human - classification; Hepatitis A Virus, Human - genetics; Hepatitis A Virus, Human - isolation & purification; Hepatitis E virus; Hepatitis E virus - classification; Hepatitis E virus - genetics; Hepatitis E virus - isolation & purification; Human viral diseases; Humans; Infant; Infants; Infectious diseases; Influenza; Influenza A; Influenza A virus; Influenza A virus - classification; Influenza A virus - genetics; Influenza A virus - isolation & purification; Medical sciences; Microbiology; Miscellaneous; Norovirus; Polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - methods; Reverse transcription; RNA Virus Infections - diagnosis; RNA Virus Infections - virology; Rotavirus; Sensitivity and Specificity; Vietnam; Viral diseases; Viral hepatitis; Virology; Viruses; Asia; Vietnam; Human; Multiplex polymerase chain reaction; Diarrhea; Hepatic disease; Orthomyxoviridae; Infant; Infection; Virus; Viral hepatitis A; Influenzavirus A; Influenza A virus; Viral disease; Digestive diseases; Intestinal disease; Diagnosis; Child; Viral hepatitis E; Reverse transcription polymerase chain reaction

A novel reverse transcription-multiplex polymerase chain reaction (RT-multiplex PCR) assay that can detect enteroviruses, hepatitis A and E viruses and influenza A virus from various hosts (avian species, human, swine and horse) was developed. The identification of that group of viruses was performe...