Improving Dengue Virus Capture Rates in Humans and Vectors in Kamphaeng Phet Province, Thailand, Using an Enhanced Spatiotemporal Surveillance Strategy

• Published in: The American journal of tropical medicine and hygiene Vol. 93; no. 1; pp. 24 - 32
• Main Authors: Thomas, Stephen J., Aldstadt, Jared, Jarman, Richard G., Buddhari, Darunee, Yoon, In-Kyu, Richardson, Jason H., Ponlawat, Alongkot, Iamsirithaworn, Sopon, Scott, Thomas W., Rothman, Alan L., Gibbons, Robert V., Lambrechts, Louis, Endy, Timothy P.
• Format: Journal Article
• Language: English
• Published: United States American Society of Tropical Medicine and Hygiene 01.07.2015; The American Society of Tropical Medicine and Hygiene
• Subjects: Adolescent; Adult; Aedes; Aged; Aged, 80 and over; Animals; Child; Child, Preschool; Culicidae; Culicidae - virology; Dengue; Dengue - epidemiology; Dengue - immunology; Dengue - virology; Dengue Virus; Dengue Virus - genetics; Dengue Virus - isolation & purification; Epidemiological Monitoring; Female; Humans; Infant; Insect Vectors; Insect Vectors - virology; Life Sciences; Male; Microbiology and Parasitology; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; RNA, Viral - analysis; Santé publique et épidémiologie; Spatio-Temporal Analysis; Thailand; Thailand - epidemiology; Virology; Young Adult; Thailand; ISEW, Thailand
• DOI: 10.4269/ajtmh.14-0242
• ISSN: 0002-9637

Dengue is of public health importance in tropical and sub-tropical regions. Dengue virus (DENV) transmission dynamics was studied in Kamphaeng Phet Province, Thailand, using an enhanced spatiotemporal surveillance of 93 hospitalized subjects with confirmed dengue (initiates) and associated cluster individuals (associates) with entomologic sampling. A total of 438 associates were enrolled from 208 houses with household members with a history of fever, located within a 200-m radius of an initiate case. Of 409 associates, 86 (21%) had laboratory-confirmed DENV infection. A total of 63 (1.8%) of the 3,565 mosquitoes collected were dengue polymerase chain reaction positive (PCR+). There was a significant relationship between spatial proximity to the initiate case and likelihood of detecting DENV from associate cases and Aedes mosquitoes. The viral detection rate from human hosts and mosquito vectors in this study was higher than previously observed by the study team in the same geographic area using different methodologies. We propose that the sampling strategy used in this study could support surveillance of DENV transmission and vector interactions.