Comparative study of Leishmania mexicana and Trypanosoma brucei NAD-dependent glycerol-3-phosphate dehydrogenase

• Published in: Molecular and biochemical parasitology Vol. 106; no. 1; pp. 83 - 91
• Main Authors: Marché, Sylvie, Michels, Paul A.M., Opperdoes, Fred R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 25.02.2000
• Subjects: Animals; Dihydroxyacetone Phosphate - metabolism; Enzyme Inhibitors - pharmacology; Enzyme Stability; Escherichia coli - enzymology; Glyceraldehyde-3-Phosphate Dehydrogenases - chemistry; Glyceraldehyde-3-Phosphate Dehydrogenases - genetics; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism; Glycerophosphates - metabolism; Glycosomal enzymes; Hydrogen-Ion Concentration; Kinetics; Leishmania mexicana; Leishmania mexicana - enzymology; Leishmania mexicana - genetics; NAD - metabolism; NAD-dependent glycerol-3-phosphate dehydrogenase; NADP - metabolism; Osmolar Concentration; Recombinant Proteins - metabolism; Sodium Chloride - pharmacology; Trypanocidal drugs; Trypanosoma brucei; Trypanosoma brucei brucei - enzymology; Glycosomal enzymes; Trypanosoma brucei; G3P, glycerol 3-phosphate; G3PDH, glycerol-3-phosphate dehydrogenase (EC 1.1.1.8); STE, sucrose–Tris–EDTA buffer; NAD-dependent glycerol-3-phosphate dehydrogenase; Trypanocidal drugs; Leishmania mexicana; DHAP, dihydroxyacetone phosphate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase

The NAD-dependent glycerol-3-phosphate dehydrogenases (G3PDH, EC 1.1.1.8) of Trypanosoma brucei and Leishmania mexicana are thought to have different roles in carbohydrate metabolism. Here the physicochemical and kinetic properties of natural G3PDH from T. brucei with the recombinant homologue of L....