Multiplex ligation-dependent probe amplification using a completely synthetic probe set

• Published in: BioTechniques Vol. 37; no. 3; pp. 399 - 405
• Main Authors: Stern, Rowena F, Roberts, Roland G, Mann, Kathy, Yau, Shu C, Berg, Jonathan, Ogilvie, Caroline Mackie
• Format: Journal Article
• Language: English
• Published: Natick, MA Future Science Ltd 01.09.2004; Eaton; Taylor & Francis Group
• Subjects: Biological and medical sciences; Chromosome Deletion; Diverse techniques; DNA Mutational Analysis - methods; DNA Probes - chemical synthesis; DNA Probes - genetics; Fundamental and applied biological sciences. Psychology; Gene Dosage; Molecular and cellular biology; Nucleic Acid Amplification Techniques - methods; Probe; Gene amplification; Ligation

The recent development of multiplex ligation-dependent probe amplification (MLPA) has provided an efficient and reliable assay for dosage screening of multiple loci in a single reaction. However, a drawback to this method is the time-consuming process of generating a probe set by cloning in single-s...