A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography–quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients

• Published in: Journal of Chromatography A Vol. 1372; pp. 110 - 119
• Main Authors: Li, Min, Tong, Xunliang, Lv, Pu, Feng, Baosheng, Yang, Li, Wu, Zheng, Cui, Xinge, Bai, Yu, Huang, Yining, Liu, Huwei
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 12.12.2014; Elsevier
• Subjects: Atherosclerosis; Biological and medical sciences; Cardiovascular system; Galactosylceramide; glucosylceramides; Human; humans; Immunity; Investigative techniques, diagnostic techniques (general aspects); isomers; lipid composition; lipid metabolism; Lipidomics; Lipids; Liquid chromatography; liquids; mass spectrometry; Medical sciences; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Patients; Profiling; Two dimensional; Two-dimensional liquid chromatography–mass spectrometry; Lipidomics; Two-dimensional liquid chromatography–mass spectrometry; Galactosylceramide; Immunity; Atherosclerosis; Biological fluid; Tandem mass spectrometry; Chemical analysis; Two dimensional chromatography; HPLC chromatography; Lipids; Cardiovascular disease; Adsorption chromatography; Electrospray; Blood; Quadrupole spectrometer; Blood plasma; Vascular disease; Time of flight mass spectroscopy; Normal phase chromatography; Clinical biology; Qualitative analysis; Two-dimensional liquid chromatography-mass spectrometry; Quantitative analysis; Human; Healthy subject; Coupled method; Sphingolipid; Patient; Glycolipid; Reversed phase chromatography; Sphingoglycolipid; Comparative study

•A not-stop-flow online NP/RP 2D LC-MS method was developed for lipid profiling.•Overall, 540 endogenous lipid species from 17 classes were identified from human plasma.•This novel method showed high sensitivity (ng/mL grade) and good repeatability.•Successful separation of isomers of GalC and GluC...