A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography–quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients
•A not-stop-flow online NP/RP 2D LC-MS method was developed for lipid profiling.•Overall, 540 endogenous lipid species from 17 classes were identified from human plasma.•This novel method showed high sensitivity (ng/mL grade) and good repeatability.•Successful separation of isomers of GalC and GluC...
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| Published in | Journal of Chromatography A Vol. 1372; pp. 110 - 119 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Amsterdam
Elsevier B.V
12.12.2014
Elsevier |
| Subjects | |
| Online Access | Get full text |
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| Abstract | •A not-stop-flow online NP/RP 2D LC-MS method was developed for lipid profiling.•Overall, 540 endogenous lipid species from 17 classes were identified from human plasma.•This novel method showed high sensitivity (ng/mL grade) and good repeatability.•Successful separation of isomers of GalC and GluC was achieved.•The levels of GalC and GluC were different in human plasma from different subjects.
A not-stop-flow online two-dimensional (2D) liquid chromatography (LC) method was developed for comprehensive lipid profiling by coupling normal- and reversed-phase LC with quadrupole time-of-flight mass spectrometry (QToF-MS), which was then applied to separate and identify the lipid species in plasma, making its merits in quality and quantity of the detection of lipids. Total 540 endogenous lipid species from 17 classes were determined in human plasma, and the differences in lipid metabolism products in human plasma between atherosclerosis patients and control subjects were explored in detail. The limit of detections (LODs) of 19 validation standards could all reach ng/mL magnitude, and the RSDs of peak area and retention time ranged 0.4–8.0% and 0.010–0.47%, respectively. In addition, a pair of isomers, galactosylceramides (GalC) and glucosylceramides (GluC), was successfully separated, showing that only the levels of GalC in atherosclerosis patients were significantly increasing, rather than GluC, compared with the controls (controls vs. patients: the ratio was 1.5–2.8-fold increasing). It would be helpful to the further research of the atherosclerosis. |
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| AbstractList | A not-stop-flow online two-dimensional (2D) liquid chromatography (LC) method was developed for comprehensive lipid profiling by coupling normal- and reversed-phase LC with quadrupole time-of-flight mass spectrometry (QToF-MS), which was then applied to separate and identify the lipid species in plasma, making its merits in quality and quantity of the detection of lipids. Total 540 endogenous lipid species from 17 classes were determined in human plasma, and the differences in lipid metabolism products in human plasma between atherosclerosis patients and control subjects were explored in detail. The limit of detections (LODs) of 19 validation standards could all reach ng/mL magnitude, and the RSDs of peak area and retention time ranged 0.4-8.0% and 0.010-0.47%, respectively. In addition, a pair of isomers, galactosylceramides (GalC) and glucosylceramides (GluC), was successfully separated, showing that only the levels of GalC in atherosclerosis patients were significantly increasing, rather than GluC, compared with the controls (controls vs. patients: the ratio was 1.5-2.8-fold increasing). It would be helpful to the further research of the atherosclerosis. •A not-stop-flow online NP/RP 2D LC-MS method was developed for lipid profiling.•Overall, 540 endogenous lipid species from 17 classes were identified from human plasma.•This novel method showed high sensitivity (ng/mL grade) and good repeatability.•Successful separation of isomers of GalC and GluC was achieved.•The levels of GalC and GluC were different in human plasma from different subjects. A not-stop-flow online two-dimensional (2D) liquid chromatography (LC) method was developed for comprehensive lipid profiling by coupling normal- and reversed-phase LC with quadrupole time-of-flight mass spectrometry (QToF-MS), which was then applied to separate and identify the lipid species in plasma, making its merits in quality and quantity of the detection of lipids. Total 540 endogenous lipid species from 17 classes were determined in human plasma, and the differences in lipid metabolism products in human plasma between atherosclerosis patients and control subjects were explored in detail. The limit of detections (LODs) of 19 validation standards could all reach ng/mL magnitude, and the RSDs of peak area and retention time ranged 0.4–8.0% and 0.010–0.47%, respectively. In addition, a pair of isomers, galactosylceramides (GalC) and glucosylceramides (GluC), was successfully separated, showing that only the levels of GalC in atherosclerosis patients were significantly increasing, rather than GluC, compared with the controls (controls vs. patients: the ratio was 1.5–2.8-fold increasing). It would be helpful to the further research of the atherosclerosis. |
| Author | Tong, Xunliang Lv, Pu Cui, Xinge Liu, Huwei Li, Min Feng, Baosheng Wu, Zheng Bai, Yu Yang, Li Huang, Yining |
| Author_xml | – sequence: 1 givenname: Min surname: Li fullname: Li, Min organization: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China – sequence: 2 givenname: Xunliang surname: Tong fullname: Tong, Xunliang organization: Department of Neurology, Peking University First Hospital, Beijing 100034, China – sequence: 3 givenname: Pu surname: Lv fullname: Lv, Pu organization: Department of Neurology, Peking University First Hospital, Beijing 100034, China – sequence: 4 givenname: Baosheng surname: Feng fullname: Feng, Baosheng organization: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China – sequence: 5 givenname: Li surname: Yang fullname: Yang, Li organization: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China – sequence: 6 givenname: Zheng surname: Wu fullname: Wu, Zheng organization: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China – sequence: 7 givenname: Xinge surname: Cui fullname: Cui, Xinge organization: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China – sequence: 8 givenname: Yu surname: Bai fullname: Bai, Yu organization: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China – sequence: 9 givenname: Yining surname: Huang fullname: Huang, Yining email: ynhuang@sina.com organization: Department of Neurology, Peking University First Hospital, Beijing 100034, China – sequence: 10 givenname: Huwei surname: Liu fullname: Liu, Huwei email: hwliu@pku.edu.cn organization: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
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| Keywords | Lipidomics Two-dimensional liquid chromatography–mass spectrometry Galactosylceramide Immunity Atherosclerosis Biological fluid Tandem mass spectrometry Chemical analysis Two dimensional chromatography HPLC chromatography Lipids Cardiovascular disease Adsorption chromatography Electrospray Blood Quadrupole spectrometer Blood plasma Vascular disease Time of flight mass spectroscopy Normal phase chromatography Clinical biology Qualitative analysis Two-dimensional liquid chromatography-mass spectrometry Quantitative analysis Human Healthy subject Coupled method Sphingolipid Patient Glycolipid Reversed phase chromatography Sphingoglycolipid Comparative study |
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| Snippet | •A not-stop-flow online NP/RP 2D LC-MS method was developed for lipid profiling.•Overall, 540 endogenous lipid species from 17 classes were identified from... A not-stop-flow online two-dimensional (2D) liquid chromatography (LC) method was developed for comprehensive lipid profiling by coupling normal- and... |
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| SubjectTerms | Atherosclerosis Biological and medical sciences Cardiovascular system Galactosylceramide glucosylceramides Human humans Immunity Investigative techniques, diagnostic techniques (general aspects) isomers lipid composition lipid metabolism Lipidomics Lipids Liquid chromatography liquids mass spectrometry Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Patients Profiling Two dimensional Two-dimensional liquid chromatography–mass spectrometry |
| Title | A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography–quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients |
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