Generating a Small Shuttle Vector for Effective Genetic Engineering of Methanosarcina mazei Allowed First Insights in Plasmid Replication Mechanism in the Methanoarchaeon

Due to their role in methane production, methanoarchaea are of high ecological relevance and genetic systems have been ever more established in the last two decades. The system for protein expression in Methanosarcina using a comprehensive shuttle vector is established; however, details about its re...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of molecular sciences Vol. 23; no. 19; p. 11910
Main Authors Thomsen, Johanna, Schmitz, Ruth A.
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.10.2022
MDPI
Subjects
archaeal plasmids
Bacteria
Chromatography
E coli
Gene deletion
Gene expression
Genetic engineering
Genetic transformation
genetics
Gram-positive bacteria
Methanosarcina
Nicking endonuclease
Plasmids
Proteins
rep type protein
Replication
rolling circle replication
shuttle vector
Size reduction
SsrA gene
Online AccessGet full text

Cover

Abstract Due to their role in methane production, methanoarchaea are of high ecological relevance and genetic systems have been ever more established in the last two decades. The system for protein expression in Methanosarcina using a comprehensive shuttle vector is established; however, details about its replication mechanism in methanoarchaea remain unknown. Here, we report on a significant optimisation of the rather large shuttle vector pWM321 (8.9 kbp) generated by Metcalf through a decrease in its size by about 35% by means of the deletion of several non-coding regions and the ssrA gene. The resulting plasmid (pRS1595) still stably replicates in M. mazei and—most likely due to its reduced size—shows a significantly higher transformation efficiency compared to pWM321. In addition, we investigate the essential gene repA, coding for a rep type protein. RepA was heterologously expressed in Escherichia coli, purified and characterised, demonstrating the significant binding and nicking activity of supercoiled plasmid DNA. Based on our findings we propose that the optimised shuttle vector replicates via a rolling circle mechanism with RepA as the initial replication protein in Methanosarcina. On the basis of bioinformatic comparisons, we propose the presence and location of a double-strand and a single-strand origin, which need to be further verified.
AbstractList Due to their role in methane production, methanoarchaea are of high ecological relevance and genetic systems have been ever more established in the last two decades. The system for protein expression in Methanosarcina using a comprehensive shuttle vector is established; however, details about its replication mechanism in methanoarchaea remain unknown. Here, we report on a significant optimisation of the rather large shuttle vector pWM321 (8.9 kbp) generated by Metcalf through a decrease in its size by about 35% by means of the deletion of several non-coding regions and the ssrA gene. The resulting plasmid (pRS1595) still stably replicates in M. mazei and—most likely due to its reduced size—shows a significantly higher transformation efficiency compared to pWM321. In addition, we investigate the essential gene repA, coding for a rep type protein. RepA was heterologously expressed in Escherichia coli, purified and characterised, demonstrating the significant binding and nicking activity of supercoiled plasmid DNA. Based on our findings we propose that the optimised shuttle vector replicates via a rolling circle mechanism with RepA as the initial replication protein in Methanosarcina. On the basis of bioinformatic comparisons, we propose the presence and location of a double-strand and a single-strand origin, which need to be further verified.
Due to their role in methane production, methanoarchaea are of high ecological relevance and genetic systems have been ever more established in the last two decades. The system for protein expression in Methanosarcina using a comprehensive shuttle vector is established; however, details about its replication mechanism in methanoarchaea remain unknown. Here, we report on a significant optimisation of the rather large shuttle vector pWM321 (8.9 kbp) generated by Metcalf through a decrease in its size by about 35% by means of the deletion of several non-coding regions and the ssrA gene. The resulting plasmid (pRS1595) still stably replicates in M. mazei and—most likely due to its reduced size—shows a significantly higher transformation efficiency compared to pWM321. In addition, we investigate the essential gene repA , coding for a rep type protein. RepA was heterologously expressed in Escherichia coli, purified and characterised, demonstrating the significant binding and nicking activity of supercoiled plasmid DNA. Based on our findings we propose that the optimised shuttle vector replicates via a rolling circle mechanism with RepA as the initial replication protein in Methanosarcina . On the basis of bioinformatic comparisons, we propose the presence and location of a double-strand and a single-strand origin, which need to be further verified.
Author Thomsen, Johanna
Schmitz, Ruth A
AuthorAffiliation Institute for General Microbiology, Christian-Albrechts-University, 24118 Kiel, Germany
AuthorAffiliation_xml – name: Institute for General Microbiology, Christian-Albrechts-University, 24118 Kiel, Germany
Author_xml – sequence: 1
  givenname: Johanna
  orcidid: 0000-0002-0183-7983
  surname: Thomsen
  fullname: Thomsen, Johanna
– sequence: 2
  givenname: Ruth A.
  orcidid: 0000-0002-6788-0829
  surname: Schmitz
  fullname: Schmitz, Ruth A.
BookMark eNpdkkuLFDEQgBtZcR969B7w4mU0j-505yIsy-w6sKK46jVUp6unM6STMcms6E_yV5pxVnGEVJ5fvoSizqsTHzxW1XNGXwmh6Gu7mRMXTLHS6KPqjNWcLyiV7ck_89PqPKUNpVzwRj2pToXkQnBWn1U_b9BjhGz9mgC5m8E5cjftcnZIvqDJIZKxxHIcy8LeI9nz2Rqy9GvrEeP-YhjJO8wT-JAgGuuBzPADLbl0LnzDgVzbmDJZ-WTXU07EevLBQZrtQD7i1llTng--KExR2DTvgTzhH2dRToDBP60ej-ASPnsYL6rP18tPV28Xt-9vVleXtwtTN11etAPtm9KpuoFatU3XKlCDbGUtBCD0nVQIogGJjNdjWwvZM9Mbw0swxkBcVKuDdwiw0dtoZ4jfdQCrf2-EuNYQSwocajWIpjUdUABZ02FUomfQcap63ktGWXG9Obi2u37GwaDPEdyR9PjE20mvw71WjVQNpUXw8kEQw9cdpqxnmww6Bx7DLmne8oapTraqoC_-QzdhF31J1Z6qeSdKhRRqcaBMDClFHP9-hlG9ryh9VFHiF86lwhA
CitedBy_id crossref_primary_10_1016_j_cej_2024_152742
crossref_primary_10_1128_aem_02247_23
Cites_doi 10.1128/jb.170.6.2575-2583.1988
10.1128/MMBR.62.2.434-464.1998
10.1128/jb.150.2.815-825.1982
10.1038/emboj.2009.125
10.1006/jmbi.2000.4042
10.1128/jb.178.11.3232-3237.1996
10.1111/j.1574-6976.2011.00265.x
10.1128/JB.00313-21
10.1371/journal.pgen.1005556
10.1196/annals.1419.019
10.1016/j.plasmid.2014.05.004
10.1093/nar/17.18.7283
10.1002/0471142727.mb0105s41
10.1016/j.mib.2005.10.010
10.1021/bi035240g
10.3390/ijms18040874
10.1007/s00438-005-1128-7
10.1016/S0021-9258(19)39392-5
10.1007/BF00693400
10.15252/msb.20209880
10.1128/jb.170.10.4979-4982.1988
10.1093/femsre/fuab004
10.1007/BF00425766
10.1016/j.cbpa.2008.01.012
10.1093/nar/20.13.3279
10.1016/0378-1119(90)90336-P
10.1038/227680a0
10.1128/genomeA.01184-14
10.1128/mBio.02766-21
10.1073/pnas.94.6.2626
10.1371/journal.pgen.1008320
10.3389/fmicb.2021.611739
10.1111/febs.15799
10.1016/j.biotechadv.2020.107668
10.1038/nrmicro1931
10.1186/s13568-017-0531-x
10.1046/j.1365-2958.1999.01498.x
10.1038/msb.2011.75
10.1016/j.biortech.2012.02.079
10.1128/JB.00596-08
10.1128/microbiolspec.PLAS-0029-2014
10.1038/nrmicro3067
10.1038/s41592-022-01488-1
10.3389/fmicb.2012.00259
10.1128/jb.179.9.2976-2986.1997
10.1111/j.1365-2958.2007.05912.x
ContentType Journal Article
Copyright 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2022 by the authors. 2022
Copyright_xml – notice: 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2022 by the authors. 2022
DBID AAYXX
CITATION
3V.
7X7
7XB
88E
8FI
8FJ
8FK
8G5
ABUWG
AFKRA
AZQEC
BENPR
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
GUQSH
K9.
M0S
M1P
M2O
MBDVC
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
Q9U
7X8
5PM
DOA
DOI 10.3390/ijms231911910
DatabaseName CrossRef
ProQuest Central (Corporate)
ProQuest Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
Research Library (Alumni Edition)
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
AUTh Library subscriptions: ProQuest Central
ProQuest One Community College
ProQuest Central
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
Research Library Prep
ProQuest Health & Medical Complete (Alumni)
Health & Medical Collection (Alumni Edition)
PML(ProQuest Medical Library)
ProQuest Research Library
Research Library (Corporate)
ProQuest - Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
ProQuest Central Basic
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals
DatabaseTitle CrossRef
Publicly Available Content Database
Research Library Prep
ProQuest Central Student
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
Research Library (Alumni Edition)
ProQuest Central China
ProQuest Central
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
ProQuest Central Korea
ProQuest Research Library
ProQuest Medical Library (Alumni)
ProQuest Central Basic
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList CrossRef

Publicly Available Content Database
Database_xml – sequence: 1
  dbid: DOA
  name: Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: BENPR
  name: AUTh Library subscriptions: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1422-0067
ExternalDocumentID oai_doaj_org_article_9d357c8a0aa640df93b1a8209b2b6101
10_3390_ijms231911910
GrantInformation_xml – fundername: Bundesministerium für Bildung und Forschung (BMBF)
  grantid: 031B0851B
GroupedDBID ---
29J
2WC
3V.
53G
5GY
5VS
7X7
88E
8FE
8FG
8FH
8FI
8FJ
8G5
A8Z
AADQD
AAFWJ
AAHBH
AAYXX
ABDBF
ABJCF
ABUWG
ACGFO
ACIHN
ACIWK
ACPRK
ADBBV
AEAQA
AENEX
AFKRA
AFPKN
AFZYC
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
CCPQU
CITATION
CS3
D1I
DIK
DU5
DWQXO
E3Z
EBD
EBS
EJD
ESTFP
ESX
F5P
FRP
FYUFA
GNUQQ
GROUPED_DOAJ
GUQSH
GX1
HCIFZ
HH5
HMCUK
HYE
IAO
ITC
KB.
KQ8
LK8
M1P
M2O
M48
M7P
MODMG
M~E
O5R
O5S
OK1
P2P
PDBOC
PIMPY
PQQKQ
PROAC
PSQYO
RIG
RNS
RPM
TR2
TUS
UKHRP
~8M
7XB
8FK
K9.
MBDVC
PQEST
PQUKI
PRINS
Q9U
7X8
5PM
ID FETCH-LOGICAL-c458t-7d0b57d0945a4975879a9d676433aeab869ea35a6e124f7436b1cbcc2bcc111a3
IEDL.DBID RPM
ISSN 1422-0067
1661-6596
IngestDate Fri Jul 05 11:14:43 EDT 2024
Tue Sep 17 21:31:09 EDT 2024
Sat Oct 05 04:54:41 EDT 2024
Thu Oct 10 20:32:08 EDT 2024
Wed Aug 07 13:57:55 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 19
Language English
License Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c458t-7d0b57d0945a4975879a9d676433aeab869ea35a6e124f7436b1cbcc2bcc111a3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0002-6788-0829
0000-0002-0183-7983
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569500/
PMID 36233214
PQID 2724283910
PQPubID 2032341
ParticipantIDs doaj_primary_oai_doaj_org_article_9d357c8a0aa640df93b1a8209b2b6101
pubmedcentral_primary_oai_pubmedcentral_nih_gov_9569500
proquest_miscellaneous_2725198679
proquest_journals_2724283910
crossref_primary_10_3390_ijms231911910
PublicationCentury 2000
PublicationDate 2022-10-01
PublicationDateYYYYMMDD 2022-10-01
PublicationDate_xml – month: 10
  year: 2022
  text: 2022-10-01
  day: 01
PublicationDecade 2020
PublicationPlace Basel
PublicationPlace_xml – name: Basel
PublicationTitle International journal of molecular sciences
PublicationYear 2022
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Smyshlyaev (ref_33) 2021; 17
Dupureur (ref_25) 2008; 12
Mirdita (ref_48) 2022; 19
Ilyina (ref_35) 1992; 20
Rother (ref_6) 2005; 8
Lilly (ref_15) 2015; 3
Laemmli (ref_44) 1970; 227
Guan (ref_22) 2014; 2
Espinosa (ref_13) 2015; 3
Erauso (ref_16) 1996; 178
Giraldo (ref_14) 1998; 62
Boer (ref_21) 2009; 28
Badel (ref_34) 2021; 45
Liu (ref_3) 2008; 1125
Chandler (ref_36) 2013; 11
Bowen (ref_26) 2003; 42
ref_39
Bron (ref_24) 1989; 17
ref_38
Soler (ref_17) 2007; 66
Zhou (ref_18) 2008; 190
Kohler (ref_10) 2012; 3
Miller (ref_43) 1988; 170
Feinbaum (ref_32) 1998; 41
Inoue (ref_42) 1990; 96
Ehlers (ref_20) 2005; 273
Sowers (ref_12) 1988; 170
Meile (ref_30) 1983; 191
Gutt (ref_45) 2021; 288
Enzmann (ref_2) 2018; 8
Weidenbach (ref_8) 2022; 204
Pfeifer (ref_4) 2020; 47
Thomas (ref_40) 1990; 265
Fink (ref_19) 2021; 12
Notredame (ref_47) 2000; 302
Hennebel (ref_7) 2012; 112
ref_28
Wood (ref_29) 1985; 142
Deppenmeier (ref_9) 2002; 4
Horinouchi (ref_23) 1982; 150
Leigh (ref_5) 2011; 35
Marsin (ref_41) 1999; 33
Sievers (ref_46) 2011; 7
Metcalf (ref_11) 1997; 94
Gao (ref_27) 2021; 12
Espinosa (ref_37) 2014; 74
Thauer (ref_1) 2008; 6
Tumbula (ref_31) 1997; 179
References_xml – volume: 170
  start-page: 2575
  year: 1988
  ident: ref_43
  article-title: A novel suicide vector and its use in construction of insertion mutations: Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
  publication-title: J. Bacteriol.
  doi: 10.1128/jb.170.6.2575-2583.1988
  contributor:
    fullname: Miller
– volume: 62
  start-page: 434
  year: 1998
  ident: ref_14
  article-title: Replication and control of circular bacterial plasmids
  publication-title: Microbiol. Mol. Biol. Rev.
  doi: 10.1128/MMBR.62.2.434-464.1998
  contributor:
    fullname: Giraldo
– volume: 150
  start-page: 815
  year: 1982
  ident: ref_23
  article-title: Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance
  publication-title: J. Bacteriol.
  doi: 10.1128/jb.150.2.815-825.1982
  contributor:
    fullname: Horinouchi
– volume: 28
  start-page: 1666
  year: 2009
  ident: ref_21
  article-title: Plasmid replication initiator RepB forms a hexamer reminiscent of ring helicases and has mobile nuclease domains
  publication-title: EMBO J.
  doi: 10.1038/emboj.2009.125
  contributor:
    fullname: Boer
– volume: 302
  start-page: 205
  year: 2000
  ident: ref_47
  article-title: T-coffee: A novel method for fast and accurate multiple sequence alignment
  publication-title: J. Mol. Biol.
  doi: 10.1006/jmbi.2000.4042
  contributor:
    fullname: Notredame
– volume: 3
  start-page: PLAS-0035-2014
  year: 2015
  ident: ref_13
  article-title: Plasmid Rolling-Circle Replication
  publication-title: Microbiol. Spectr.
  contributor:
    fullname: Espinosa
– volume: 178
  start-page: 3232
  year: 1996
  ident: ref_16
  article-title: Sequence of plasmid pGT5 from the archaeon Pyrococcus abyssi: Evidence for rolling-circle replication in a hyperthermophile
  publication-title: J. Bacteriol.
  doi: 10.1128/jb.178.11.3232-3237.1996
  contributor:
    fullname: Erauso
– volume: 35
  start-page: 577
  year: 2011
  ident: ref_5
  article-title: Model organisms for genetics in the domain Archaea: Methanogens, halophiles, Thermococcales and Sulfolobales
  publication-title: FEMS Microbiol. Rev.
  doi: 10.1111/j.1574-6976.2011.00265.x
  contributor:
    fullname: Leigh
– volume: 204
  start-page: 128
  year: 2022
  ident: ref_8
  article-title: Small Proteins in Archaea, a Mainly Unexplored World
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.00313-21
  contributor:
    fullname: Weidenbach
– ident: ref_39
  doi: 10.1371/journal.pgen.1005556
– volume: 1125
  start-page: 171
  year: 2008
  ident: ref_3
  article-title: Metabolic, phylogenetic, and ecological diversity of the methanogenic archaea
  publication-title: Ann. N. Y. Acad. Sci.
  doi: 10.1196/annals.1419.019
  contributor:
    fullname: Liu
– volume: 74
  start-page: 15
  year: 2014
  ident: ref_37
  article-title: Bringing them together: Plasmid pMV158 rolling circle replication and conjugation under an evolutionary perspective
  publication-title: Plasmid
  doi: 10.1016/j.plasmid.2014.05.004
  contributor:
    fullname: Espinosa
– volume: 17
  start-page: 7283
  year: 1989
  ident: ref_24
  article-title: Similarity of minus origins of replication and flanking open reading frames of plasmids pUB110, pTB913 and pMV158
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/17.18.7283
  contributor:
    fullname: Bron
– volume: 41
  start-page: 19
  year: 1998
  ident: ref_32
  article-title: Introduction to Plasmid Biology
  publication-title: Curr. Protoc. Mol. Biol.
  doi: 10.1002/0471142727.mb0105s41
  contributor:
    fullname: Feinbaum
– volume: 8
  start-page: 745
  year: 2005
  ident: ref_6
  article-title: Genetic technologies for Archaea
  publication-title: Curr. Opin. Microbiol.
  doi: 10.1016/j.mib.2005.10.010
  contributor:
    fullname: Rother
– volume: 42
  start-page: 12643
  year: 2003
  ident: ref_26
  article-title: Investigation of restriction enzyme cofactor requirements: A relationship between metal ion properties and sequence specificity
  publication-title: Biochemistry
  doi: 10.1021/bi035240g
  contributor:
    fullname: Bowen
– volume: 4
  start-page: 453
  year: 2002
  ident: ref_9
  article-title: The genome of Methanosarcina mazei: Evidence for lateral gene transfer between bacteria and archaea
  publication-title: J. Mol. Microbiol. Biotechnol.
  contributor:
    fullname: Deppenmeier
– ident: ref_28
  doi: 10.3390/ijms18040874
– volume: 273
  start-page: 290
  year: 2005
  ident: ref_20
  article-title: Development of genetic methods and construction of a chromosomal glnK1 mutant in Methanosarcina mazei strain Gö1
  publication-title: Mol. Genet. Genom.
  doi: 10.1007/s00438-005-1128-7
  contributor:
    fullname: Ehlers
– volume: 265
  start-page: 5519
  year: 1990
  ident: ref_40
  article-title: In vitro studies of the initiation of staphylococcal plasmid replication. Specificity of RepD for its origin (oriD) and characterization of the Rep-ori tyrosyl ester intermediate
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(19)39392-5
  contributor:
    fullname: Thomas
– volume: 142
  start-page: 259
  year: 1985
  ident: ref_29
  article-title: A newly-isolated marine methanogen harbors a small cryptic plasmid
  publication-title: Arch. Microbiol.
  doi: 10.1007/BF00693400
  contributor:
    fullname: Wood
– volume: 17
  start-page: e9880
  year: 2021
  ident: ref_33
  article-title: Sequence analysis of tyrosine recombinases allows annotation of mobile genetic elements in prokaryotic genomes
  publication-title: Mol. Syst. Biol.
  doi: 10.15252/msb.20209880
  contributor:
    fullname: Smyshlyaev
– volume: 170
  start-page: 4979
  year: 1988
  ident: ref_12
  article-title: Plasmid DNA from the acetotrophic methanogen Methanosarcina acetivorans
  publication-title: J. Bacteriol.
  doi: 10.1128/jb.170.10.4979-4982.1988
  contributor:
    fullname: Sowers
– volume: 45
  start-page: 9658
  year: 2021
  ident: ref_34
  article-title: Archaeal tyrosine recombinases
  publication-title: FEMS Microbiol. Rev.
  doi: 10.1093/femsre/fuab004
  contributor:
    fullname: Badel
– volume: 191
  start-page: 480
  year: 1983
  ident: ref_30
  article-title: A plasmid in the archaebacterium Methanobacterium thermoautotrophicum
  publication-title: Mol. Genet. Genom.
  doi: 10.1007/BF00425766
  contributor:
    fullname: Meile
– volume: 12
  start-page: 250
  year: 2008
  ident: ref_25
  article-title: Roles of metal ions in nucleases
  publication-title: Curr. Opin. Chem. Biol.
  doi: 10.1016/j.cbpa.2008.01.012
  contributor:
    fullname: Dupureur
– volume: 20
  start-page: 3279
  year: 1992
  ident: ref_35
  article-title: Conserved sequence motifs in the initiator proteins for rolling circle DNA replication encoded by diverse replicons from eubacteria, eucaryotes and archaebacteria
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/20.13.3279
  contributor:
    fullname: Ilyina
– volume: 96
  start-page: 23
  year: 1990
  ident: ref_42
  article-title: High efficiency transformation of Escherichia coli with plasmids
  publication-title: Gene
  doi: 10.1016/0378-1119(90)90336-P
  contributor:
    fullname: Inoue
– volume: 227
  start-page: 680
  year: 1970
  ident: ref_44
  article-title: Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
  publication-title: Nature
  doi: 10.1038/227680a0
  contributor:
    fullname: Laemmli
– volume: 2
  start-page: e01184-14
  year: 2014
  ident: ref_22
  article-title: Draft Genome Sequence of an Obligately Methylotrophic Methanogen, Methanococcoides methylutens, Isolated from Marine Sediment
  publication-title: Genome Announc.
  doi: 10.1128/genomeA.01184-14
  contributor:
    fullname: Guan
– volume: 12
  start-page: e02766-21
  year: 2021
  ident: ref_19
  article-title: A Shuttle-Vector System Allows Heterologous Gene Expression in the Thermophilic Methanogen Methanothermobacter thermautotrophicus ΔH
  publication-title: mBio
  doi: 10.1128/mBio.02766-21
  contributor:
    fullname: Fink
– volume: 94
  start-page: 2626
  year: 1997
  ident: ref_11
  article-title: A genetic system for Archaea of the genus Methanosarcina: Liposome-mediated transformation and construction of shuttle vectors
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.94.6.2626
  contributor:
    fullname: Metcalf
– ident: ref_38
  doi: 10.1371/journal.pgen.1008320
– volume: 12
  start-page: 23
  year: 2021
  ident: ref_27
  article-title: Putative Extracellular Electron Transfer in Methanogenic Archaea
  publication-title: Front. Microbiol.
  doi: 10.3389/fmicb.2021.611739
  contributor:
    fullname: Gao
– volume: 288
  start-page: 5350
  year: 2021
  ident: ref_45
  article-title: High complexity of Glutamine synthetase regulation in Methanosarcina mazei: Small protein 26 interacts and enhances glutamine synthetase activity
  publication-title: FEBS J.
  doi: 10.1111/febs.15799
  contributor:
    fullname: Gutt
– volume: 47
  start-page: 107668
  year: 2020
  ident: ref_4
  article-title: Archaea Biotechnology
  publication-title: Biotechnol. Adv.
  doi: 10.1016/j.biotechadv.2020.107668
  contributor:
    fullname: Pfeifer
– volume: 6
  start-page: 579
  year: 2008
  ident: ref_1
  article-title: Methanogenic archaea: Ecologically relevant differences in energy conservation
  publication-title: Nat. Rev. Microbiol.
  doi: 10.1038/nrmicro1931
  contributor:
    fullname: Thauer
– volume: 8
  start-page: 1
  year: 2018
  ident: ref_2
  article-title: Methanogens: Biochemical background and biotechnological applications
  publication-title: AMB Express
  doi: 10.1186/s13568-017-0531-x
  contributor:
    fullname: Enzmann
– volume: 33
  start-page: 537
  year: 1999
  ident: ref_41
  article-title: The active site of the rolling circle replication protein Rep75 is involved in site-specific nuclease, ligase and nucleotidyl transferase activities
  publication-title: Mol. Microbiol.
  doi: 10.1046/j.1365-2958.1999.01498.x
  contributor:
    fullname: Marsin
– volume: 7
  start-page: 539
  year: 2011
  ident: ref_46
  article-title: Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega
  publication-title: Mol. Syst. Biol.
  doi: 10.1038/msb.2011.75
  contributor:
    fullname: Sievers
– volume: 112
  start-page: 1
  year: 2012
  ident: ref_7
  article-title: Methanosarcina: The rediscovered methanogen for heavy duty biomethanation
  publication-title: Bioresour. Technol.
  doi: 10.1016/j.biortech.2012.02.079
  contributor:
    fullname: Hennebel
– volume: 190
  start-page: 5710
  year: 2008
  ident: ref_18
  article-title: Precise Determination, Cross-Recognition, and Functional Analysis of the Double-Strand Origins of the Rolling-Circle Replication Plasmids in Haloarchaea
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.00596-08
  contributor:
    fullname: Zhou
– volume: 3
  start-page: PLAS-0029-2014
  year: 2015
  ident: ref_15
  article-title: Mechanisms of Theta Plasmid Replication
  publication-title: Microbiol. Spectr.
  doi: 10.1128/microbiolspec.PLAS-0029-2014
  contributor:
    fullname: Lilly
– volume: 11
  start-page: 525
  year: 2013
  ident: ref_36
  article-title: Breaking and joining single-stranded DNA: The HUH endonuclease superfamily
  publication-title: Nat. Rev. Microbiol.
  doi: 10.1038/nrmicro3067
  contributor:
    fullname: Chandler
– volume: 19
  start-page: 679
  year: 2022
  ident: ref_48
  article-title: ColabFold: Making protein folding accessible to all
  publication-title: Nat. Methods
  doi: 10.1038/s41592-022-01488-1
  contributor:
    fullname: Mirdita
– volume: 3
  start-page: 259
  year: 2012
  ident: ref_10
  article-title: Genetic manipulation of Methanosarcina spp.
  publication-title: Front. Microbiol.
  doi: 10.3389/fmicb.2012.00259
  contributor:
    fullname: Kohler
– volume: 179
  start-page: 2976
  year: 1997
  ident: ref_31
  article-title: Characterization of pURB500 from the archaeon Methanococcus maripaludis and construction of a shuttle vector
  publication-title: J. Bacteriol.
  doi: 10.1128/jb.179.9.2976-2986.1997
  contributor:
    fullname: Tumbula
– volume: 66
  start-page: 357
  year: 2007
  ident: ref_17
  article-title: The rolling-circle plasmid pTN1 from the hyperthermophilic archaeon Thermococcus nautilus
  publication-title: Mol. Microbiol.
  doi: 10.1111/j.1365-2958.2007.05912.x
  contributor:
    fullname: Soler
SSID ssj0023259
Score 2.408634
Snippet Due to their role in methane production, methanoarchaea are of high ecological relevance and genetic systems have been ever more established in the last two...
SourceID doaj
pubmedcentral
proquest
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
StartPage 11910
SubjectTerms archaeal plasmids
Bacteria
Chromatography
E coli
Gene deletion
Gene expression
Genetic engineering
Genetic transformation
genetics
Gram-positive bacteria
Methanosarcina
Nicking endonuclease
Plasmids
Proteins
rep type protein
Replication
rolling circle replication
shuttle vector
Size reduction
SsrA gene
SummonAdditionalLinks – databaseName: Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Ni9UwFA0yILgRP_HpKFcQd2XapklflqP4GIURYRyZXblpE6by2g72PUR_kr_Se5N2eF25cdEumpCmvUnuOfk4V4g3mfdNZmviJrmnG42NCRa1ThpTa1Z40h55vuP8sz67LD5dqauDUF-8JyzKA8cfd2Iaqcp6jSmiLtLGG2kzJLdlbG7J9Ufik6mZTE1US-YhTFpG3ifRyuiorimJ4J-037uRQI1hZbN04Y2CaP8CaS73SR44ns0DcX9CjHAaa_pQ3HH9I3E3xpD89Vj8icLRvHsZEC463G7h4nrP0sTwLUzJA-FSiCrFNLQB56ei4ECJEAYP544n0YeRYwv1CB3-di2cbrfDT9fApiWQCB_7kan8CG0PXwh1d20DBODnaT8qgo8Rt2PHGQhYzmUGPSY39E_E5ebD1_dnyRSAIakLtd4lZZNaRTdTKCwMMYvSoGl0SShGokO71sahVKgdoQRPWETbrLZ1ndNFYyjKp-KoH3r3TACvBpYoy1pbWfjcGuWNc0VhyaIoZboSb2dDVDdRZ6MifsIWqxYWW4l3bKbbTCyPHR5Qo6mmRlP9q9GsxPFs5Grqs2OVlzmrz4V3vL5Npt7GSyjYu2Ef8hDkZZHClSgXjWNRoWVK314H3W6iokal6fP_8QUvxL2cD2KEbYXH4mj3Y-9eEjza2VehJ_wFjWsQmQ
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: AUTh Library subscriptions: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagFRIXxFMEChokxC1qEjvO-oRa1FVBalVRinqLxo5DgzZJaXaF4CfxK5nJY2kuHOJDPHIijT3zzdj-Roi3cVkWsXUUmyQlNWQbQ1ROh4VxmhmedImc7zg51ccX6tNlejkm3LrxWOVkE3tDXbSOc-T7SZYwNxh5t_fXP0KuGsW7q2MJjbtiN4kVb9PuHh6dnn3ehlwy6culxeSFQp0aPbBsSgr096vvdUfgxjDDWTTzSj15_wxxzs9L3nJAy4fiwYgc4WBQ9SNxxzePxb2hluSvJ-LPQCDNp5gB4bzG1QrOrzZMUQxf-9Q8ED6Fga2YTBywPA0FtxgJoS3hxHMyve24xlCDUONvX8HBatX-9AUsKwKL8LHpOKTvoGrgjNB3XRVAQH5K_9EQfJ246moWIIA5jdnzMvm2eSoulkdfPhyHYyGG0Kl0sQ6zIrIpNUalqAxFGJlBU-iM0IxEj3ahjUeZovaEFkrCJNrGzjqX0EO2FOUzsdO0jX8ugHcFM5SZ01aqMrEmLY33StmiNChlFIh3kyLy64FvI6c4hTWWzzQWiENW01aIabL7F-3Nt3xcdbkpZJq5BUaIWkX0CWljJMxjbGIJN8aB2JuUnI9rt8v_zbRAvNl206rjrRRsfLvpZQj6MllhILLZ5Jj90Lynqa56_m4KSU0aRS_-__GX4n7CVy36g4N7Ymd9s_GvCACt7etxlv8Fa0gJ4Q
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Journals (Open Access)
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ba9RAFB6kRemLeMW1VY4gvkWTzGSy8yCliksVVoS60rdwJpm0kVzsZhdtf1J_peckm7VBn3zIPGRuIWcu3zeX7wjxMsjzLLApcZMwp4DGRg9Vqr3MpJoVnnSOvN4x_6yPF-rTaXT6R1Jo8wPbf1I79ie1WJavf11cHlKHf8uMkyj7m-J71RJMMaxVRux9N1RScWOfq-2GAuGGyPQSm39n2RN3aBiX7LJnNDt1Iv4j5Dk-N3ljIprdE3c3CBKOepPfF7dc_UDc7n1KXj4U172QNJ9mBoSTCssSTs7XLFUM37oleiCcCr1qMQ11wOmpKLihTAhNDnPHi-pNy76GaoQKr1wBR2XZ_HQZzAoCjfCxbpnat1DU8IVQeFVkQIB-WAakIvhacdFWnICA5lBmp8_kmvqRWMw-fH1_7G0cMnipiqYrL858G1FgVITKENOIDZpMx4RqJDq0U20cygi1I9SQEzbRNkhtmob00JiK8rHYqZvaPRHAu4MxyjjVVqo8tCbKjXNK2Sw3KKU_Ea8GQyQ_et2NhPgKGy8ZGW8i3rGZtolYLrt70SzPkk3vS0wmozidoo-olU9VSBsgYR9jQ0v4MZiIg8HIydAEkzAOWY2uq-PFNpp6H2-pYO2adZeGIDCLFk5EPGocow8ax9TFeafjTdTURL7_9L9z7ou9kG9jdGcLD8TOarl2zwgjrezzrvX_BpqtFtE
  priority: 102
  providerName: Scholars Portal
Title Generating a Small Shuttle Vector for Effective Genetic Engineering of Methanosarcina mazei Allowed First Insights in Plasmid Replication Mechanism in the Methanoarchaeon
URI https://www.proquest.com/docview/2724283910
https://search.proquest.com/docview/2725198679
https://pubmed.ncbi.nlm.nih.gov/PMC9569500
https://doaj.org/article/9d357c8a0aa640df93b1a8209b2b6101
Volume 23
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELbaIiQuiKdYKJGRELd0s7ETx8e26lKQtlpRivYW2Y7TBiVO1ewKwU_iVzLjJFVz5ZA5JI5tafz4Zjz-hpCPi7IsFtqAbRKXIGBtDBU3aVhIkyLDU1oq9HesLtLzK_51k2z2SDLehfFB-0ZXR65ujlx142MrbxszH-PE5uvVKWB6mUTRfJ_sC8ZGE32wshgA-p5Mk4E9P69-Nh1gGIlEZpj2DRZshsl5JvuQp-ufYMxphOSDLWf5jDwdsCI97vv0nOxZ94I87rNH_n5J_vaU0Ri3TBW9bFRd08ubHZIS0x_eGU8BkdKenxgWNYrloSr6gIOQtiVdWXSftx1mFXKKNuqPrehxXbe_bEGXFcBD-sV1aMR3tHJ0DXi7qQoK0H10-EEVeIG46hosAJByrNMzMdnWvSJXy7Pvp-fhkHohNDzJtqEoIp2AkDxRXIJNIaSSRSoAvzBllc5SaRVLVGoBH5SAQlK9MNqYGB5YPRV7TQ5c6-wbQvEcUCgmTKoZL2Mtk1Jay7kuSqkYi2bk06iI_LZn2MjBMkHl5RPlzcgJqum-EBJj-xft3XU-DI9cFiwRJlORUimPoAmmFwpQjtSxBqS4mJHDUcn5MFu7PBYx8s75Nj7cf4Z5hocnytl258sA2EV6whkRk8Ex6dD0Cwxgz9g9DNi3__3nO_IkxnsXPorwkBxs73b2PaChrQ5gDmwEyGz5OSCPTs4u1t8C71kIcJdKQK54Fvg58g9_5xWy
link.rule.ids 230,315,733,786,790,870,891,2115,2236,12083,12792,21416,24346,27955,27956,31752,31753,33406,33407,33777,33778,43343,43633,43838,53825,53827,74100,74390,74657
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Nb9QwELWgCMEF8SkCBYyEuEVNYsdZn1BBLFvoVkhtUW_R2HHaoE1Sml0h-En8SmacZGkuHOJDPHIijT3zZmy_YexNXJZFbCzGJkmJDdrGEKRVYaGtIoYnVQLlO5ZHanEqP5-lZ0PCrRuOVY420RvqorWUI99LsoS4wdC7vbv8EVLVKNpdHUpo3GS3pFCC5vls_mkbcInEF0uL0QeFKtWq59gUGObvVd_rDqGNJn6zaOKTPHX_BG9OT0tecz_z--zegBv5fq_oB-yGax6y230lyV-P2J-ePprOMHPgxzWsVvz4YkMExfybT8xzRKe85ypGA8dJHofi1_gIeVvypaNUettRhaEGeA2_XcX3V6v2pyv4vEKoyA-ajgL6jlcN_4rYu64KjjB-TP7hEHSZuOpqEkB4OY7pWZlc2zxmp_OPJx8W4VCGIbQyna3DrIhMio2WKUiN8UWmQRcqQywjwIGZKe1ApKAcYoUSEYkysTXWJvigJQXxhO00beOeMk57ghmIzCojZJkYnZbaOSlNUWoQIgrY21ER-WXPtpFjlEIayycaC9h7UtNWiEiy_Yv26jwf1lyuC5FmdgYRgJIRfkKYGBDxaJMYRI1xwHZHJefDyu3yf_MsYK-33bjmaCMFGtduvAwCX6IqDFg2mRyTH5r2NNWFZ-_GgFSnUfTs_x9_xe4sTpaH-eHB0Zfn7G5Cly78EcJdtrO-2rgXCIXW5qWf738Bm4ULaA
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagCMQF8VRDCxgJcYs2iRNnfULlsbRAq0qlqLdo7Ng0aJO0za4Q_CR-JTNOsjQXDvEhHjmRxh5_Mx5_w9ir2Lky1gZ9k8Rhg7YxhNTIsFRGEsOTdEDxjsMjuX-afjrLzob8p25IqxxtojfUZWsoRj5L8oS4wXB3m7khLeL4_eLNxWVIFaTopHUop3GT3SKQTWUc5ouPG-dLJL5wWoz7USgzJXu-TYGCs-pH3SHMUcR1Fk32J0_jP8Ge08zJa1vR4j67N2BIvtcr_QG7YZuH7HZfVfLXI_anp5KmfGYO_KSG5ZKfnK-JrJh_80F6jkiV97zFaOw4yeNQ_Bo3IW8dP7QUVm87qjbUAK_ht6343nLZ_rQlX1QIG_lB05Fz3_Gq4ceIw-uq5Ajpx0AgDkEXi6uuJgGEmuOYnqHJts1jdrr48PXdfjiUZAhNms1XYV5GOsNGpRmkCn2NXIEqZY64RoAFPZfKgshAWsQNDvUgdWy0MQk-aFVBPGFbTdvYbcbpfDAHkRupReoSrTKnrE1TXToFQkQBez0qorjomTcK9FhIY8VEYwF7S2raCBFhtn_RXn0vhvVXqFJkuZlDBCDTCD8hdAyIfpRONCLIOGC7o5KLYRV3xb85F7CXm25cf3SoAo1t114GQTDRFgYsn0yOyQ9Ne5rq3DN5o3Oqsih6-v-Pv2B3cKoXXw6OPu-wuwndv_DZhLtsa3W1ts8QFa30cz_d_wLu3w-d
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Generating+a+Small+Shuttle+Vector+for+Effective+Genetic+Engineering+of+Methanosarcina+mazei+Allowed+First+Insights+in+Plasmid+Replication+Mechanism+in+the+Methanoarchaeon&rft.jtitle=International+journal+of+molecular+sciences&rft.au=Thomsen%2C+Johanna&rft.au=Schmitz%2C+Ruth+A.&rft.date=2022-10-01&rft.pub=MDPI&rft.eissn=1422-0067&rft.volume=23&rft.issue=19&rft_id=info:doi/10.3390%2Fijms231911910&rft_id=info%3Apmid%2F36233214&rft.externalDBID=PMC9569500
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1422-0067&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1422-0067&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1422-0067&client=summon