Molecular cloning and biochemical characterization of a novel cytochrome P450, flavone synthase II, that catalyzes direct conversion of flavanones to flavones

• Published in: Plant and cell physiology Vol. 40; no. 11; pp. 1182 - 1186
• Main Authors: Akashi, T. (Nihon Univ., Tokyo (Japan)), Fukuchi Mizutani, M, Aoki, T, Ueyama, Y, Yonekura Sakakibara, K, Tanaka, Y, Kusumi, T, Ayabe, S
• Format: Journal Article
• Language: English
• Published: Japan Oxford University Press 01.11.1999
• Subjects: (2S)-flavanone 2-hydroxylase; Amino Acid Sequence; Antirrhinum majus; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; Catalysis; cDNA cloning; CITOCROMO P450; CLONACION MOLECULAR; CLONAGE MOLECULAIRE; Cloning, Molecular; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; CYTOCHROME P450; flavanones; flavone synthase; Flavone synthase II; flavones; Flavonoid biosynthesis; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; Flavonoids - biosynthesis; Flavonoids - metabolism; Mixed Function Oxygenases - genetics; Mixed Function Oxygenases - metabolism; MOLECULAR CLONING; Molecular Sequence Data; Phylogeny; Plants - enzymology; Plants - genetics; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Sequence Homology, Amino Acid; Snapdragon; Substrate Specificity; Torenia
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a029505

Cytochrome P450 cDNAs, AFNS2 and TFNS5, were isolated from snapdragon and torenia petal cDNA libraries, respectively, based on the sequence homology with licorice CYP93B1 cDNA encoding (2S)-flavanone 2-hydroxylase. They were expressed in yeast and identified to encode flavone synthase II catalyzing direct conversion of flavanones to flavones probably via 2-hydroxyflavanones.