Large-scaled human serum sphingolipid profiling by using reversed-phase liquid chromatography coupled with dynamic multiple reaction monitoring of mass spectrometry: Method development and application in hepatocellular carcinoma - Li, Jia; Hu, Chunxiu; Zhao, Xinjie; Dai, Weidong; Chen, Shili; Lu, Xin; Xu, Guowang (Journal of Chromatography A, 2013)
Keywords: alkaline hydrolysis; bioactive properties; Biological and medical sciences; biomarkers; blood serum; Carcinoma, Hepatocellular - blood; cell membranes; ceramides; Chromatography, Reverse-Phase - methods; detection limit; hepatoma; homeostasis; Host-tumor relations. Immunology. Biological markers; human diseases; Humans; Lipidomics; Liquid chromatography–mass spectrometry; Liver Neoplasms - blood; mass spectrometry; Medical sciences; monitoring; Multiple reaction monitoring; Reproducibility of Results; reversed-phase liquid chromatography; screening; Serum; Sphingolipid profiling; Sphingolipids - blood; sphingomyelins; Tandem Mass Spectrometry - methods; Tert-butyl methyl ether; Tumors; Tert-butyl methyl ether; Sphingolipid profiling; Multiple reaction monitoring; MRM; Lipidomics; Sph; APCI; dHCer; MTBE; Cer; RPLC; HexCer; LacCer; Serum; SM; ESI; Liquid chromatography–mass spectrometry; NPLC; HILIC; Ultra performance liquid chromatography; Biological fluid; Tandem mass spectrometry; Chemical analysis; Biological marker; Lipids; Hepatic disease; Hepatocellular carcinoma; Solvent extraction; Electrospray; Blood; Sample preparation; Quantitative analysis; Human; Validation; Healthy subject; Liquid liquid extraction; Coupled method; Multiple reaction monitoring mode; Liquid chromatography-mass spectrometry; Sphingolipid; Patient; Malignant tumor; Reversed phase chromatography; Digestive diseases; Blood serum; Chemometrics; Cancer; Principal component analysis; electrospray ionization; ceramide; hydrophilic interaction liquid chromatography; reversed phase liquid chromatography; normal phase liquid chromatography; hexosylceramide; dihydroceramide; lactosylceramide; free sphingoid base; atmospheric-pressure chemical ionization; sphingomyelin