Visualizing and discovering cellular structures with super-resolution microscopy
Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction limit—in light microscopy and enabled visualization of previously invisible molecular details in biological systems. Since their conception, super-resolution imaging methods have continually evolved and can now b...
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| Published in | Science (American Association for the Advancement of Science) Vol. 361; no. 6405; pp. 880 - 887 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
The American Association for the Advancement of Science
31.08.2018
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0036-8075 1095-9203 1095-9203 |
| DOI | 10.1126/science.aau1044 |
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| Abstract | Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction limit—in light microscopy and enabled visualization of previously invisible molecular details in biological systems. Since their conception, super-resolution imaging methods have continually evolved and can now be used to image cellular structures in three dimensions, multiple colors, and living systems with nanometer-scale resolution. These methods have been applied to answer questions involving the organization, interaction, stoichiometry, and dynamics of individual molecular building blocks and their integration into functional machineries in cells and tissues. In this Review, we provide an overview of super-resolution methods, their state-of-the-art capabilities, and their constantly expanding applications to biology, with a focus on the latter. We will also describe the current technical challenges and future advances anticipated in super-resolution imaging. |
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| AbstractList | Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction limit—in light microscopy and enabled visualization of previously invisible molecular details in biological systems. Since their conception, super-resolution imaging methods have continually evolved and can now be used to image cellular structures in three dimensions, multiple colors, and living systems with nanometer-scale resolution. These methods have been applied to answer questions involving the organization, interaction, stoichiometry, and dynamics of individual molecular building blocks and their integration into functional machineries in cells and tissues. In this Review, we provide an overview of super-resolution methods, their state-of-the-art capabilities, and their constantly expanding applications to biology, with a focus on the latter. We will also describe the current technical challenges and future advances anticipated in super-resolution imaging. Super-resolution microscopy has overcome a long-held resolution barrier-the diffraction limit-in light microscopy and enabled visualization of previously invisible molecular details in biological systems. Since their conception, super-resolution imaging methods have continually evolved and can now be used to image cellular structures in three dimensions, multiple colors, and living systems with nanometer-scale resolution. These methods have been applied to answer questions involving the organization, interaction, stoichiometry, and dynamics of individual molecular building blocks and their integration into functional machineries in cells and tissues. In this Review, we provide an overview of super-resolution methods, their state-of-the-art capabilities, and their constantly expanding applications to biology, with a focus on the latter. We will also describe the current technical challenges and future advances anticipated in super-resolution imaging.Super-resolution microscopy has overcome a long-held resolution barrier-the diffraction limit-in light microscopy and enabled visualization of previously invisible molecular details in biological systems. Since their conception, super-resolution imaging methods have continually evolved and can now be used to image cellular structures in three dimensions, multiple colors, and living systems with nanometer-scale resolution. These methods have been applied to answer questions involving the organization, interaction, stoichiometry, and dynamics of individual molecular building blocks and their integration into functional machineries in cells and tissues. In this Review, we provide an overview of super-resolution methods, their state-of-the-art capabilities, and their constantly expanding applications to biology, with a focus on the latter. We will also describe the current technical challenges and future advances anticipated in super-resolution imaging. |
| Author | Zhuang, Xiaowei Zhou, Ruobo Sigal, Yaron M. |
| AuthorAffiliation | 1 Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA |
| AuthorAffiliation_xml | – name: 1 Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA |
| Author_xml | – sequence: 1 givenname: Yaron M. orcidid: 0000-0001-9636-4164 surname: Sigal fullname: Sigal, Yaron M. organization: Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA – sequence: 2 givenname: Ruobo orcidid: 0000-0001-8628-0282 surname: Zhou fullname: Zhou, Ruobo organization: Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA – sequence: 3 givenname: Xiaowei orcidid: 0000-0002-6034-7853 surname: Zhuang fullname: Zhuang, Xiaowei organization: Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30166485$$D View this record in MEDLINE/PubMed |
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| Snippet | Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction limit—in light microscopy and enabled visualization of previously... Super-resolution microscopy has overcome a long-held resolution barrier-the diffraction limit-in light microscopy and enabled visualization of previously... Super-resolution microscopy has overcome a long-held resolution barrier – the diffraction limit – in light microscopy and enabled visualization of previously... |
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| SubjectTerms | Animals Cells - ultrastructure Diffraction Humans Imaging, Three-Dimensional - methods Light microscopy Microscopy, Fluorescence - methods Molecular biology Molecular Imaging - methods Neurons - ultrastructure Scientific imaging Synapses - ultrastructure |
| Title | Visualizing and discovering cellular structures with super-resolution microscopy |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/30166485 https://www.proquest.com/docview/2097636746 https://www.proquest.com/docview/2098770245 https://pubmed.ncbi.nlm.nih.gov/PMC6535400 |
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