Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing - Li, Yifan; Lin, Zhenquan; Huang, Can; Zhang, Yan; Wang, Zhiwen; Tang, Ya-jie; Chen, Tao; Zhao, Xueming (Metabolic engineering, 2015)
Keywords: base pair mismatch; batch fermentation; beta Carotene - biosynthesis; beta-carotene; biochemical pathways; Clustered Regularly Interspaced Short Palindromic Repeats - genetics; Combinatorial metabolic engineering; CRISPR-Cas Systems; CRISPR/Cas9; DNA Mismatch Repair; DNA repair; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; genetic variation; genome; Genome editing; Genome, Bacterial; Metabolic Engineering; RNA Editing - genetics; β-Carotene; CRISPR; ORF; TRMR; CRISPR/Cas9; G3P; gRNA; Combinatorial metabolic engineering; β-Carotene; MAGE; DSB; MMR; MEP; PEP; Genome editing; PAM; ssDNA; dsDNA