Cell-Free Production of Aggregation-Prone Proteins in Soluble and Active Forms

• Published in: Biotechnology progress Vol. 21; no. 5; pp. 1412 - 1419
• Main Authors: Kang, Sang-Hyeon, Kim, Dong-Myung, Kim, Hyo-Jin, Jun, Soo-Youn, Lee, Ki-Young, Kim, Hye-Jin
• Format: Journal Article
• Language: English
• Published: USA American Chemical Society 01.09.2005; American Institute of Chemical Engineers
• Subjects: Biological and medical sciences; Biotechnology; Cell-Free System; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Inclusion Bodies - chemistry; Inclusion Bodies - metabolism; Molecular Chaperones - chemistry; Molecular Chaperones - metabolism; Protein Disulfide-Isomerases - chemistry; Protein Disulfide-Isomerases - metabolism; Protein Engineering - methods; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Aggregation; Soluble form; Protein; Production

We have developed an efficient cell‐free protein synthesis system for the production of soluble and active eukaryotic proteins that are predominantly produced as inclusion bodies in bacteria. S30 extracts (indicating the supernatant of cell homogenate when centrifuged at 30,000g) for cell‐free prote...