Autofluorescence lifetime flow cytometry with time‐correlated single photon counting

• Published in: Cytometry. Part A Vol. 105; no. 8; pp. 607 - 620
• Main Authors: Samimi, Kayvan, Pasachhe, Ojaswi, Guzman, Emmanuel Contreras, Riendeau, Jeremiah, Gillette, Amani A., Pham, Dan L., Wiech, Kasia J., Moore, Darcie L., Skala, Melissa C.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.08.2024; Wiley Subscription Services, Inc
• Subjects: Animals; CD28 antigen; CD3 antigen; Channel gating; Cost analysis; Flow cytometry; Flow Cytometry - methods; Flow geometry; Fluorescence; fluorescence lifetime; Humans; Image processing; Information processing; Jurkat Cells; label‐free sensing; Lasers; Lymphocytes; Lymphocytes T; Metabolism; Mice; Microfluidics; Microscopes; Microscopy; NAD(P)H; Neural stem cells; Optical Imaging - methods; Phasors; Photomultiplier tubes; Photons; Scanning microscopy; Semiconductor lasers; Single-Cell Analysis - methods; single‐cell analysis; Sodium cyanide; Stem cells; Temporal resolution; time tagger; fluorescence lifetime; time tagger; label‐free sensing; NAD(P)H; flow cytometry; metabolism; single‐cell analysis

Autofluorescence lifetime imaging microscopy (FLIM) is sensitive to metabolic changes in single cells based on changes in the protein‐binding activities of the metabolic co‐enzymes NAD(P)H. However, FLIM typically relies on time‐correlated single‐photon counting (TCSPC) detection electronics on lase...