Quantitative determination of intracellular Asulacrine in MCF-7 breast cancer cells by liquid chromatography-mass spectrometry and its application to cellular pharmacokinetic studies of P188 modified liposomes

• Published in: Biomedical chromatography Vol. 30; no. 12; pp. 1908 - 1914
• Main Authors: Zang, Xiaojie, Zhang, Jingwei, Zhou, Yaqian, Chen, Qianying, Peng, Ying, Sun, Jianguo, Liu, Jiali, Liu, Wenyue, Wang, Guangji, Zhou, Fang
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.12.2016
• Subjects: Amsacrine - analogs & derivatives; Amsacrine - pharmacokinetics; Antineoplastic Agents - pharmacokinetics; asulacrine; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Chromatography, Liquid - methods; Female; Humans; intracellular concentration; LC/MS; liposome; Liposomes; Mass Spectrometry - methods; MCF-7 Cells; Reproducibility of Results; LC/MS; asulacrine; intracellular concentration; liposome

Asulacrine (ASL), an analogue of amsacrine, has shown higher anti‐breast and anti‐lung cancer activity. Hereby, a new sensitive and selective liquid chromatography–mass spectrometry (LC/MS) method was developed to determine intracellular asulacrine. The chromatographic separation was performed on an...