The HIV-1 Nef Protein and Phagocyte NADPH Oxidase Activation

• Published in: The Journal of biological chemistry Vol. 277; no. 44; pp. 42136 - 42143
• Main Authors: Vilhardt, Frederik, Plastre, Olivier, Sawada, Makoto, Suzuki, Kazuo, Wiznerowicz, Maciej, Kiyokawa, Etsuko, Trono, Didier, Krause, Karl-Heinz
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 01.11.2002
• Subjects: Amino Acid Motifs; Animals; Cell Line; Enzyme Activation; Gene Products, nef - physiology; HIV-1 - physiology; Mice; Microglia - enzymology; NADPH Oxidases - metabolism; nef Gene Products, Human Immunodeficiency Virus; Oncogene Proteins - physiology; Phagocytes - enzymology; Protein Subunits; Proto-Oncogene Proteins c-vav; rac1 GTP-Binding Protein - physiology; src Homology Domains; Superoxides - metabolism

Nef, a multifunctional HIV protein, activates the Vav/Rac/p21-activated kinase (PAK) signaling pathway. Given the potential role of this pathway in the activation of the phagocyte NADPH oxidase, we have investigated the effect of the HIV-1 Nef protein on the phagocyte respiratory burst. Microglia (c...