Heat shock transcription factor HSF1 is required for survival of sensory hair cells against acoustic overexposure

• Published in: Hearing research Vol. 182; no. 1; pp. 88 - 96
• Main Authors: Sugahara, Kazuma, Inouye, Sachiye, Izu, Hanae, Katoh, Yumiko, Katsuki, Kensaku, Takemoto, Tsuyoshi, Shimogori, Hiroaki, Yamashita, Hiroshi, Nakai, Akira
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.08.2003; Elsevier
• Subjects: Animals; Biological and medical sciences; Blotting, Western; Cell death; Cell Death - physiology; DNA-Binding Proteins - physiology; Evoked Potentials, Auditory, Brain Stem; Female; Guinea Pigs; Hair cells; Hair Cells, Auditory - pathology; Hair Cells, Auditory - physiopathology; Hearing Loss, Noise-Induced - prevention & control; Heat shock factor; Heat shock protein; Heat Shock Transcription Factors; Hot Temperature - adverse effects; Hyperthermia; Immunohistochemistry; Male; Medical sciences; Mice; Neuropharmacology; Neuroprotective agent; Noise - adverse effects; Noise injury; Pharmacology. Drug treatments; Transcription Factors - physiology; Hsp, heat shock protein; PBS, phosphate-buffered saline; Cell death; Hair cells; Heat shock protein; ABR, auditory brainstem response; Heat shock factor; HSF, heat shock transcription factor; PFA, paraformaldehyde; Hyperthermia; Noise injury; Corti organ; Heat shock protein: Hyperthermia; Cardiovascular disease; Inner ear; Organ of hearing; Guinea pig; ENT disease; Noise pollution; Transcription factor; Hair cell of the ear; Internal ear disease; Rodentia; Gene expression; Transcription factor HSF1; Survival; Acoustic trauma; Vertebrata; Mammalia; Mouse; Animal; Cochlea
• ISSN: 0378-5955; 1878-5891
• DOI: 10.1016/S0378-5955(03)00180-1

To analyze the role of heat shock response in the cochleae, we induced major heat shock proteins, Hsp70, Hsp90, and Hsp27 by perfusion of hot saline into the middle ear cavity (called ‘local heat shock’ in guinea pigs. Hsps were induced in almost all of the cochlear cells including the sensory hair cells in the organ of Corti. We showed that loss of both the sensory hair cells and the auditory function induced by acoustic overexposure was inhibited by pretreatment of the inner ear with local heat shock. To examine the role of heat shock transcription factor 1(HSF), which activates heat shock genes in response to heat shock, in the protection of sensory hair cells, we analyzed acoustic injury in HSF1-null mice. We found that the loss of sensory hair cells was more significant in HSF1-null mice compared with that of wild-type mice when mice were subjected to acoustic overexposure. These results indicate that HSF1 is required for survival of the sensory hair cells against acoustic overexposure.