Aqueous Tear Deficiency Increases Conjunctival Interferon-γ (IFN-γ) Expression and Goblet Cell Loss

• Published in: Investigative ophthalmology & visual science Vol. 56; no. 12; pp. 7545 - 7550
• Main Authors: Pflugfelder, Stephen C., De Paiva, Cintia S., Moore, Quianta L., Volpe, Eugene A., Li, De-Quan, Gumus, Koray, Zaheer, Mahira L., Corrales, Rosa M.
• Format: Journal Article
• Language: English
• Published: United States The Association for Research in Vision and Ophthalmology 01.11.2015
• Subjects: Adult; Aged; Aged, 80 and over; Cells, Cultured; Conjunctiva - metabolism; Conjunctiva - pathology; Cornea; Dry Eye Syndromes - genetics; Dry Eye Syndromes - metabolism; Dry Eye Syndromes - pathology; Female; Fluorescent Antibody Technique, Indirect; Gene Expression Regulation; Goblet Cells - metabolism; Goblet Cells - pathology; Humans; Interferon-gamma - biosynthesis; Interferon-gamma - genetics; Male; Middle Aged; Real-Time Polymerase Chain Reaction; RNA - genetics; Tears - metabolism; Young Adult

To investigate the hypothesis that increased interferon-γ (IFN-γ) expression is associated with conjunctival goblet cell loss in subjects with tear dysfunction. Goblet cell density (GCD) was measured in impression cytology from the temporal bulbar conjunctiva, and gene expression was measured in cyt...