Mutation analysis of the cross-reactive epitopes of Japanese encephalitis virus envelope glycoprotein

• Published in: Journal of general virology Vol. 93; no. 6; pp. 1185 - 1192
• Main Authors: Chiou, Shyan-Song, Fan, Yi-Chin, Crill, Wayne D., Chang, Ruey-Yi, Chang, Gwong-Jen J.
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.06.2012
• Subjects: Amino Acid Sequence; amino acid substitution; amino acids; Antibodies, Viral - immunology; Biological and medical sciences; cross reaction; Cross Reactions; DNA Mutational Analysis; Encephalitis Virus, Japanese - chemistry; Encephalitis Virus, Japanese - genetics; Encephalitis Virus, Japanese - immunology; Encephalitis, Japanese - immunology; Encephalitis, Japanese - virology; Epitope Mapping; epitopes; Epitopes - chemistry; Epitopes - genetics; Epitopes - immunology; Flavivirus; Fundamental and applied biological sciences. Psychology; genes; glycoproteins; Humans; Japanese encephalitis virus; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - genetics; Membrane Glycoproteins - immunology; Microbiology; Miscellaneous; Molecular Sequence Data; monoclonal antibodies; mutants; plasmids; site-directed mutagenesis; Viral Envelope Proteins - chemistry; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Virology; virus-like particles; Virus; Antigenic determinant; Glycoprotein; Japanese encephalitis virus; Japanese encephalitis group virus; Mutation; Flaviviridae; Flavivirus

Group and serocomplex cross-reactive epitopes have been identified in the envelope (E) protein of several flaviviruses and have proven critical in vaccine and diagnostic antigen development. Here, we performed site-directed mutagenesis across the E gene of a recombinant expression plasmid that encod...