A statistical approach to determining responses to individual peptides from pooled-peptide ELISpot data

• Published in: Journal of immunological methods Vol. 435; pp. 43 - 49
• Main Authors: Ström, Peter, Støer, Nathalie, Borthwick, Nicola, Dong, Tao, Hanke, Tomáš, Reilly, Marie
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2016
• Subjects: AIDS Vaccines - immunology; Algorithms; Background adjustment; Data Accuracy; ELISpot assay; EM-algorithm; Enzyme-Linked Immunospot Assay - methods; HIV-1 - immunology; Human immunodeficiency virus 1; Humans; immune response; immune system; Internet; Leukocytes, Mononuclear - immunology; Likelihood Functions; Male; Peptide pools; peptides; Peptides - analysis; Peptides - immunology; Poisson Distribution; scientists; statistical analysis; vaccination; vaccines; Peptide pools; Background adjustment; EM-algorithm; ELISpot assay

To investigate in detail the effect of infection or vaccination on the human immune system, ELISpot assays are used to simultaneously test the immune response to a large number of peptides of interest. Scientists commonly use “peptide pools”, where, instead of an individual peptide, a test well cont...