Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data

• Published in: Neuroscience letters Vol. 339; no. 1; pp. 62 - 66
• Main Authors: Ramakers, Christian, Ruijter, Jan M, Deprez, Ronald H.Lekanne, Moorman, Antoon F.M
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 13.03.2003; Elsevier
• Subjects: Biological and medical sciences; Calibration; Comparative Ct method; DNA, Complementary - analysis; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; General aspects. Models. Methods; Linear Models; Linear regression; mRNA quantification; Organic Chemicals; PCR efficiency; Polymerase Chain Reaction - standards; Polymerase Chain Reaction - statistics & numerical data; Quantitative polymerase chain reaction (PCR); Real-time PCR; RNA, Messenger - analysis; Standard curve method; SYBR ® green I; Time Factors; Vertebrates: nervous system and sense organs; Quantitative polymerase chain reaction (PCR); Comparative C t method; SYBR ® green I; Real-time PCR; mRNA quantification; Linear regression; PCR efficiency; Standard curve method; Comparative C, method; SYBR® green I; Real time; Polymerase chain reaction; Messenger RNA; Technique; Quantitative analysis

Quantification of mRNAs using real-time polymerase chain reaction (PCR) by monitoring the product formation with the fluorescent dye SYBR Green I is being extensively used in neurosciences, developmental biology, and medical diagnostics. Most PCR data analysis procedures assume that the PCR efficien...