Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data - Ramakers, Christian; Ruijter, Jan M; Deprez, Ronald H.Lekanne; Moorman, Antoon F.M (Neuroscience letters, 2003)
Keywords: Biological and medical sciences; Calibration; Comparative Ct method; DNA, Complementary - analysis; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; General aspects. Models. Methods; Linear Models; Linear regression; mRNA quantification; Organic Chemicals; PCR efficiency; Polymerase Chain Reaction - standards; Polymerase Chain Reaction - statistics & numerical data; Quantitative polymerase chain reaction (PCR); Real-time PCR; RNA, Messenger - analysis; Standard curve method; SYBR ® green I; Time Factors; Vertebrates: nervous system and sense organs; Quantitative polymerase chain reaction (PCR); Comparative C t method; SYBR ® green I; Real-time PCR; mRNA quantification; Linear regression; PCR efficiency; Standard curve method; Comparative C, method; SYBR® green I; Real time; Polymerase chain reaction; Messenger RNA; Technique; Quantitative analysis