Computationally designed libraries for rapid enzyme stabilization

• Published in: Protein engineering, design and selection Vol. 27; no. 2; pp. 49 - 58
• Main Authors: Wijma, Hein J., Floor, Robert J., Jekel, Peter A., Baker, David, Marrink, Siewert J., Janssen, Dick B.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2014
• Subjects: Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Computer applications; Computer Simulation; Disulfides - chemistry; Enzyme Stability; Epoxide Hydrolases - chemistry; Epoxide Hydrolases - genetics; Epoxide Hydrolases - metabolism; Models, Molecular; Mutagenesis, Site-Directed; Original; Protein Conformation; Rhodococcus - chemistry; Rhodococcus - enzymology; Rhodococcus - genetics; Temperature; enzyme stability; screening; protein stability engineering; thermostability; design; in silico screening; in silico design

The ability to engineer enzymes and other proteins to any desired stability would have wide-ranging applications. Here, we demonstrate that computational design of a library with chemically diverse stabilizing mutations allows the engineering of drastically stabilized and fully functional variants o...