Functional expression of lepidopteran-selective neurotoxin in baculovirus: Potential for effective pest management
Recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. Recently we identified and characterized a novel lepidopteran-selective toxin, Buthus tamulus insect...
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| Published in | Biochimica et biophysica acta Vol. 1760; no. 2; pp. 158 - 163 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
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Netherlands
Elsevier B.V
01.02.2006
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| Online Access | Get full text |
| ISSN | 0304-4165 0006-3002 1872-8006 |
| DOI | 10.1016/j.bbagen.2005.11.008 |
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| Abstract | Recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. Recently we identified and characterized a novel lepidopteran-selective toxin,
Buthus tamulus insect-selective toxin (ButaIT), having 37 amino acids and eight half cysteine residues from the venom of the South Indian red scorpion,
Mesobuthus tamulus. The synthetic toxin gene containing the ButaIT sequence in frame to the bombyxin signal sequence was engineered into a polyhedrin positive
Autographa californica nuclear polyhedrosis virus (AcMNPV) genome under the control of the p10 promoter. Toxin expression in the haemolymph of infected larvae of
Heliothis virescens and also in an insect cell culture system was confirmed by western blot analysis using antibody raised against the GST-ButaIT fusion protein. The recombinant NPV (ButaIT-NPV) showed enhanced insecticidal activity on the larvae of
Heliothis virescens as evidenced by a significant reduction in median survival time (ST
50) and also a greater reduction in feeding damage as compared to the wild-type AcMNPV. |
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| AbstractList | Recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. Recently we identified and characterized a novel lepidopteran-selective toxin,
Buthus tamulus insect-selective toxin (ButaIT), having 37 amino acids and eight half cysteine residues from the venom of the South Indian red scorpion,
Mesobuthus tamulus. The synthetic toxin gene containing the ButaIT sequence in frame to the bombyxin signal sequence was engineered into a polyhedrin positive
Autographa californica nuclear polyhedrosis virus (AcMNPV) genome under the control of the p10 promoter. Toxin expression in the haemolymph of infected larvae of
Heliothis virescens and also in an insect cell culture system was confirmed by western blot analysis using antibody raised against the GST-ButaIT fusion protein. The recombinant NPV (ButaIT-NPV) showed enhanced insecticidal activity on the larvae of
Heliothis virescens as evidenced by a significant reduction in median survival time (ST
50) and also a greater reduction in feeding damage as compared to the wild-type AcMNPV. Recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. Recently we identified and characterized a novel lepidopteran-selective toxin, Buthus tamulus insect-selective toxin (ButaIT), having 37 amino acids and eight half cysteine residues from the venom of the South Indian red scorpion, Mesobuthus tamulus. The synthetic toxin gene containing the ButaIT sequence in frame to the bombyxin signal sequence was engineered into a polyhedrin positive Autographa californica nuclear polyhedrosis virus (AcMNPV) genome under the control of the p10 promoter. Toxin expression in the haemolymph of infected larvae of Heliothis virescens and also in an insect cell culture system was confirmed by western blot analysis using antibody raised against the GST-ButaIT fusion protein. The recombinant NPV (ButaIT-NPV) showed enhanced insecticidal activity on the larvae of Heliothis virescens as evidenced by a significant reduction in median survival time (ST50) and also a greater reduction in feeding damage as compared to the wild-type AcMNPV.Recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. Recently we identified and characterized a novel lepidopteran-selective toxin, Buthus tamulus insect-selective toxin (ButaIT), having 37 amino acids and eight half cysteine residues from the venom of the South Indian red scorpion, Mesobuthus tamulus. The synthetic toxin gene containing the ButaIT sequence in frame to the bombyxin signal sequence was engineered into a polyhedrin positive Autographa californica nuclear polyhedrosis virus (AcMNPV) genome under the control of the p10 promoter. Toxin expression in the haemolymph of infected larvae of Heliothis virescens and also in an insect cell culture system was confirmed by western blot analysis using antibody raised against the GST-ButaIT fusion protein. The recombinant NPV (ButaIT-NPV) showed enhanced insecticidal activity on the larvae of Heliothis virescens as evidenced by a significant reduction in median survival time (ST50) and also a greater reduction in feeding damage as compared to the wild-type AcMNPV. Recombinant baculovirus expressing insect-selective neurotoxins derived from venomous animals are considered as an attractive alternative to chemical insecticides for efficient insect control agents. Recently we identified and characterized a novel lepidopteran-selective toxin, Buthus tamulus insect-selective toxin (ButaIT), having 37 amino acids and eight half cysteine residues from the venom of the South Indian red scorpion, Mesobuthus tamulus. The synthetic toxin gene containing the ButaIT sequence in frame to the bombyxin signal sequence was engineered into a polyhedrin positive Autographa californica nuclear polyhedrosis virus (AcMNPV) genome under the control of the p10 promoter. Toxin expression in the haemolymph of infected larvae of Heliothis virescens and also in an insect cell culture system was confirmed by western blot analysis using antibody raised against the GST-ButaIT fusion protein. The recombinant NPV (ButaIT-NPV) showed enhanced insecticidal activity on the larvae of Heliothis virescens as evidenced by a significant reduction in median survival time (ST50) and also a greater reduction in feeding damage as compared to the wild-type AcMNPV. |
| Author | Hackett, Kevin J. Buckley, Ellen Rajendra, Wudayagiri Hammock, Bruce D. |
| Author_xml | – sequence: 1 givenname: Wudayagiri surname: Rajendra fullname: Rajendra, Wudayagiri email: rajendraw2k@yahoo.co.in organization: Division of Molecular Biology, Department of Zoology, Sri Venkateswara University, Tirupati-517 502, India – sequence: 2 givenname: Kevin J. surname: Hackett fullname: Hackett, Kevin J. organization: USDA, ARS, National Program Staff, National Program Leader for Beneficial Insects, 5601 Sunnyside Avenue, Building 4, Room 4-2228, Beltsville, MD 20705-5139, USA – sequence: 3 givenname: Ellen surname: Buckley fullname: Buckley, Ellen organization: Insect Biocontrol Laboratory, USDA, ARS, Beltsville, MD 20705, USA – sequence: 4 givenname: Bruce D. surname: Hammock fullname: Hammock, Bruce D. organization: Department of Entomology and Cancer Research Center, University of California, Davis, CA 95616, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16406338$$D View this record in MEDLINE/PubMed |
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| Keywords | ST 50 Heliothis virescens Scorpion venom Buta-IT GST AcMNPV Lepidopteran-selective toxin Recombinant biopesticide Baculovirus LD 50 Mesobuthus tamulus Integrated pest management |
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| SubjectTerms | Animals Baculoviridae - metabolism Baculovirus Biological Assay Genetic Engineering Heliothis virescens Integrated pest management Lepidoptera - drug effects Lepidopteran-selective toxin Mesobuthus tamulus Neurotoxins - biosynthesis Neurotoxins - genetics Pest Control, Biological - methods Recombinant biopesticide Recombinant Fusion Proteins - pharmacology Scorpion venom Scorpion Venoms - biosynthesis Scorpion Venoms - genetics Transfection |
| Title | Functional expression of lepidopteran-selective neurotoxin in baculovirus: Potential for effective pest management |
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