Isolation and characterization of tri- and tetra-repeat microsatellite loci in the white-spotted charr Salvelinus leucomaenis (Salmonidae)

Tri‐ and tetra‐motif repeat microsatellite marker loci were developed for the white‐spotted charr Salvelinus leucomaenis. The 454 pyrosequencing was used to discover repeat arrays, and eight microsatellite‐primer sets, available for the estimation of polymorphisms, were identified. The number of all...

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Published inJournal of fish biology Vol. 86; no. 3; pp. 1199 - 1202
Main Authors Yamamoto, S., Sekino, M.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.03.2015
Wiley Subscription Services, Inc
Subjects
454 pyrosequencing
Alleles
Animals
Heterozygote
Microsatellite Repeats
next-generation sequencer
polymorphism
Polymorphism, Genetic
Sequence Analysis, DNA
short tandem repeat
simple sequence repeat
Trout - genetics
454 pyrosequencing
polymorphism
short tandem repeat
next-generation sequencer
simple sequence repeat
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Summary:Tri‐ and tetra‐motif repeat microsatellite marker loci were developed for the white‐spotted charr Salvelinus leucomaenis. The 454 pyrosequencing was used to discover repeat arrays, and eight microsatellite‐primer sets, available for the estimation of polymorphisms, were identified. The number of alleles in a wild population ranged from two to four and the observed and expected heterozygosities were 0·180–0·600 and 0·188–0·599, respectively.
Bibliography:ArticleID:JFB12628
istex:3DFB6FC343217503F6CF2AE3112F00F54720F236
ark:/67375/WNG-1G1DV5VT-L
Japan Society for the Promotion of Science
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1112
1095-8649
DOI:10.1111/jfb.12628