Development of a novel dengue virus serotype-specific multiplex real-time reverse transcription-polymerase chain reaction assay for blood screening

• Published in: Transfusion (Philadelphia, Pa.) Vol. 56; no. 12; pp. 3094 - 3100
• Main Authors: Tezuka, Kenta, Kuramitsu, Madoka, Okuma, Kazu, Nojima, Kiyoko, Araki, Kumiko, Shinohara, Naoya, Matsumoto, Chieko, Satake, Masahiro, Takasaki, Tomohiko, Saijo, Masayuki, Kurane, Ichiro, Hamaguchi, Isao
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.12.2016; Wiley Subscription Services, Inc
• Subjects: Blood Safety; Dengue - diagnosis; Dengue - prevention & control; Dengue - transmission; Dengue fever; Dengue Virus - genetics; Genes; Humans; Multiplex Polymerase Chain Reaction; Polymerase chain reaction; Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction; RNA, Viral - analysis; RNA, Viral - blood; Serogroup; Transfusion Reaction

BACKGROUND Dengue fever is caused by four related RNA viruses of the genus Flavivirus, dengue virus (DENV)‐1, ‐2, ‐3, and ‐4, which are transmitted to humans by mosquitoes. Although DENV is not endemic in Japan, an autochthonous dengue outbreak occurred in 2014. Several transfusion‐transmitted cases...