CRISPR Primer Designer： Design primers for knockout and chromosome imaging CRISPR-Cas system

• Published in: Journal of integrative plant biology Vol. 57; no. 7; pp. 613 - 617
• Main Authors: Yan, Meng, Zhou, Shi-Rong, Xue, Hong-Wei
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Blackwell Publishing Ltd 01.07.2015; National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, the Chinese Academy of Sciences,Shanghai 200032, China
• Subjects: biologists; Cas系统; Chromosome imaging; chromosomes; Chromosomes, Plant - genetics; Clustered Regularly Interspaced Short Palindromic Repeats - genetics; CRISPR; CRISPR-Cas Systems - genetics; DNA Primers - metabolism; epigenetics; Gene Knockout Techniques; genes; image analysis; Imaging, Three-Dimensional; Internet; knockout; plasmids; primer; researchers; Software; 基因敲除; 基因组结构; 多参数分析; 引物设计; 成像系统; 染色体; 设计工具; knockout; CRISPR; software; primer; Chromosome imaging

The clustered regularly interspaced short palin- dromic repeats （CRiSPR）-associated system enables biologists to edit genomes precisely and provides a powerful tool for perturbing endogenous gene regulation, modulation of epigenetic markers, and genome architecture. However, there are concerns about...