Unlocking autofluorescence in the era of full spectrum analysis: Implications for immunophenotype discovery projects

• Published in: Cytometry. Part A Vol. 101; no. 11; pp. 922 - 941
• Main Authors: Jameson, Vanta J., Luke, Tina, Yan, Yuting, Hind, Angela, Evrard, Maximilien, Man, Kevin, Mackay, Laura K., Kallies, Axel, Villadangos, Jose A., McWilliam, Hamish E. G., Perez‐Gonzalez, Alexis
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.11.2022; Wiley Subscription Services, Inc
• Subjects: Abundance; Algorithms; alveolar macrophages; autofluorescence; autofluorescence discovery; autofluorescence extraction; Coloring Agents; Cytometry; Design of experiments; Empirical analysis; Experimental design; Fluorescence; fluorochrome brightness; full spectrum unmixing; Homeostasis; Immunology; immunophenotype discovery; Immunophenotyping; instrument sensitivity; label‐free cytometry; Original; Signal detection; signal resolution; Spectrum analysis; instrument sensitivity; label-free cytometry; signal resolution; alveolar macrophages; fluorochrome brightness; autofluorescence extraction; immunophenotype discovery; autofluorescence discovery; full spectrum unmixing; autofluorescence

Understanding the complex elements affecting signal resolution in cytometry is key for quality experimental design and data. In this study, we incorporate autofluorescence as a contributing factor to our understanding of resolution in cytometry and corroborate its impact in fluorescence signal detec...