In silico prediction and validation of the importance of the Entner-Doudoroff pathway in poly(3-hydroxybutyrate) production by metabolically engineered Escherichia coli

• Published in: Biotechnology and bioengineering Vol. 83; no. 7; pp. 854 - 863
• Main Authors: Hong, Soon Ho, Park, Si Jae, Moon, Soo Yun, Park, Jong Pil, Lee, Sang Yup
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 30.09.2003; Wiley
• Subjects: 2-keto-3-deoxy-6-phosphogluconate aldolase; Alcaligenes - genetics; Biological and medical sciences; Biotechnology; Citric Acid Cycle; Entner-Doudoroff pathway; Escherichia coli; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli - metabolism; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetic Engineering; Genetic technics; Hydroxybutyrates - metabolism; metabolic flux analysis; Metabolism; Methods. Procedures. Technologies; Modification of gene expression level; Mutation; Neural Networks (Computer); Operon; Plasmids; poly(3-hydroxybutyrate); Polyesters - metabolism; Predictive Value of Tests; Recombinant Proteins - metabolism; Reproducibility of Results; Recombinant microorganism; metabolic flux analysis; Escherichia coli; Ester polymer; Butyrate(hydroxy)polymer; Metabolic pathway; Metabolism; 2-keto-3-deoxy-6-phosphogluconate aldolase; Entner-Doudoroff pathway; Production; poly(3-hydroxybutyrate); Bacteria; Metabolic engineering; Enterobacteriaceae

The metabolic network of Escherichia coli was constructed and was used to simulate the distribution of metabolic fluxes in wild‐type E. coli and recombinant E. coli producing poly(3‐hydroxybutyrate) [P(3HB)]. The flux of acetyl‐CoA into the tricarboxylic acid (TCA) cycle, which competes with the P(3...