Isolation and characterization of embryonic stem-like cells from canine blastocysts

• Published in: Molecular reproduction and development Vol. 73; no. 3; pp. 298 - 305
• Main Authors: Hatoya, Shingo, Torii, Ryuzo, Kondo, Yasushi, Okuno, Tsuyoshi, Kobayashi, Kinji, Wijewardana, Viskam, Kawate, Noritoshi, Tamada, Hiromichi, Sawada, Tsutomu, Kumagai, Daijiro, Sugiura, Kikuya, Inaba, Toshio
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.03.2006; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; blastocyst; Blastocyst - cytology; Blastocyst - physiology; canine; Cell Differentiation - physiology; Cell Separation - methods; Cells, Cultured; Cloning, Organism - methods; Coculture Techniques; Dogs; Embryo, Mammalian - cytology; Embryo, Mammalian - physiology; Embryology: invertebrates and vertebrates. Teratology; Embryonic Development - physiology; embryonic stem cell; Fibroblasts - cytology; Fundamental and applied biological sciences. Psychology; isolation; Mice; Molecular embryology; Morula - cytology; Morula - physiology; Stem Cells - cytology; Stem Cells - physiology; Fissipedia; Insulation; Carnivora; Embryo; Stem cell; Blastocyst; Biological marker; canine; isolation; Gene expression; In vitro; Characterization; Vertebrata; Mammalia; embryonic stem cell; Morphological analysis; Dog

Embryonic stem (ES) cells are pluripotent cells with the capacity to generate any type of cell. Here we describe the isolation of ES‐like cells from canine blastocysts. Canine embryos were collected from beagle bitches at day 11–16 of first estrus. A total of 80 normal embryos were obtained from 15...