TRPM7 silencing attenuates Mg2+ influx in cardiac myoblasts, H9c2 cells

TRPM7, a member of the melastatin subfamily of transient receptor potential channels, is suggested to be a potential candidate for a physiological Mg 2+ channel. However, there is no direct evidence of Mg 2+ permeation through endogenous TRPM7. To determine the physiological roles of TRPM7 in intrac...

Full description

Saved in:
Bibliographic Details
Published inThe journal of physiological sciences Vol. 70; no. 1; pp. 1 - 47
Main Authors Tashiro, Michiko, Konishi, Masato, Kobayashi, Ryo, Inoue, Hana, Yokoyama, Utako
Format Journal Article
LanguageEnglish
Published Heidelberg Springer 07.10.2020
BioMed Central
Subjects
Online AccessGet full text

Cover

Loading…
Abstract TRPM7, a member of the melastatin subfamily of transient receptor potential channels, is suggested to be a potential candidate for a physiological Mg 2+ channel. However, there is no direct evidence of Mg 2+ permeation through endogenous TRPM7. To determine the physiological roles of TRPM7 in intracellular Mg 2+ homeostasis, we measured the cytoplasmic free Mg 2+ concentration ([Mg 2+ ] i ) in TRPM7-silenced H9c2 cells. [Mg 2+ ] i was measured in a cluster of 8–10 cells using the fluorescent indicator, furaptra. TRPM7 silencing did not change [Mg 2+ ] i in Ca 2+ -free Tyrode’s solution containing 1 mM Mg 2+ . Increasing the extracellular Mg 2+ to 92.5 mM raised [Mg 2+ ] i in control cells (1.56 ± 0.19 mM) at 30 min, while this effect was significantly attenuated in TRPM7-silenced cells (1.12 ± 0.07 mM). The Mg 2+ efflux driven by Na + gradient was unaffected by TRPM7 silencing. These results suggest that TRPM7 regulates the rate of Mg 2+ influx in H9c2 cells, although cytoplasmic Mg 2+ homeostasis at basal conditions is unaffected by TRPM7 silencing.
AbstractList TRPM7, a member of the melastatin subfamily of transient receptor potential channels, is suggested to be a potential candidate for a physiological Mg.sup.2+ channel. However, there is no direct evidence of Mg.sup.2+ permeation through endogenous TRPM7. To determine the physiological roles of TRPM7 in intracellular Mg.sup.2+ homeostasis, we measured the cytoplasmic free Mg.sup.2+ concentration ([Mg.sup.2+].sub.i) in TRPM7-silenced H9c2 cells. [Mg.sup.2+].sub.i was measured in a cluster of 8-10 cells using the fluorescent indicator, furaptra. TRPM7 silencing did not change [Mg.sup.2+].sub.i in Ca.sup.2+-free Tyrode's solution containing 1 mM Mg.sup.2+. Increasing the extracellular Mg.sup.2+ to 92.5 mM raised [Mg.sup.2+].sub.i in control cells (1.56 [+ or -] 0.19 mM) at 30 min, while this effect was significantly attenuated in TRPM7-silenced cells (1.12 [+ or -] 0.07 mM). The Mg.sup.2+ efflux driven by Na.sup.+ gradient was unaffected by TRPM7 silencing. These results suggest that TRPM7 regulates the rate of Mg.sup.2+ influx in H9c2 cells, although cytoplasmic Mg.sup.2+ homeostasis at basal conditions is unaffected by TRPM7 silencing. Keywords: Magnesium, TRPM7, Cardiac myoblast, H9c2, Mag-fura-2
TRPM7, a member of the melastatin subfamily of transient receptor potential channels, is suggested to be a potential candidate for a physiological Mg.sup.2+ channel. However, there is no direct evidence of Mg.sup.2+ permeation through endogenous TRPM7. To determine the physiological roles of TRPM7 in intracellular Mg.sup.2+ homeostasis, we measured the cytoplasmic free Mg.sup.2+ concentration ([Mg.sup.2+].sub.i) in TRPM7-silenced H9c2 cells. [Mg.sup.2+].sub.i was measured in a cluster of 8-10 cells using the fluorescent indicator, furaptra. TRPM7 silencing did not change [Mg.sup.2+].sub.i in Ca.sup.2+-free Tyrode's solution containing 1 mM Mg.sup.2+. Increasing the extracellular Mg.sup.2+ to 92.5 mM raised [Mg.sup.2+].sub.i in control cells (1.56 [+ or -] 0.19 mM) at 30 min, while this effect was significantly attenuated in TRPM7-silenced cells (1.12 [+ or -] 0.07 mM). The Mg.sup.2+ efflux driven by Na.sup.+ gradient was unaffected by TRPM7 silencing. These results suggest that TRPM7 regulates the rate of Mg.sup.2+ influx in H9c2 cells, although cytoplasmic Mg.sup.2+ homeostasis at basal conditions is unaffected by TRPM7 silencing.
TRPM7, a member of the melastatin subfamily of transient receptor potential channels, is suggested to be a potential candidate for a physiological Mg 2+ channel. However, there is no direct evidence of Mg 2+ permeation through endogenous TRPM7. To determine the physiological roles of TRPM7 in intracellular Mg 2+ homeostasis, we measured the cytoplasmic free Mg 2+ concentration ([Mg 2+ ] i ) in TRPM7-silenced H9c2 cells. [Mg 2+ ] i was measured in a cluster of 8–10 cells using the fluorescent indicator, furaptra. TRPM7 silencing did not change [Mg 2+ ] i in Ca 2+ -free Tyrode’s solution containing 1 mM Mg 2+ . Increasing the extracellular Mg 2+ to 92.5 mM raised [Mg 2+ ] i in control cells (1.56 ± 0.19 mM) at 30 min, while this effect was significantly attenuated in TRPM7-silenced cells (1.12 ± 0.07 mM). The Mg 2+ efflux driven by Na + gradient was unaffected by TRPM7 silencing. These results suggest that TRPM7 regulates the rate of Mg 2+ influx in H9c2 cells, although cytoplasmic Mg 2+ homeostasis at basal conditions is unaffected by TRPM7 silencing.
TRPM7, a member of the melastatin subfamily of transient receptor potential channels, is suggested to be a potential candidate for a physiological Mg2+ channel. However, there is no direct evidence of Mg2+ permeation through endogenous TRPM7. To determine the physiological roles of TRPM7 in intracellular Mg2+ homeostasis, we measured the cytoplasmic free Mg2+ concentration ([Mg2+]i) in TRPM7-silenced H9c2 cells. [Mg2+]i was measured in a cluster of 8–10 cells using the fluorescent indicator, furaptra. TRPM7 silencing did not change [Mg2+]i in Ca2+-free Tyrode’s solution containing 1 mM Mg2+. Increasing the extracellular Mg2+ to 92.5 mM raised [Mg2+]i in control cells (1.56 ± 0.19 mM) at 30 min, while this effect was significantly attenuated in TRPM7-silenced cells (1.12 ± 0.07 mM). The Mg2+ efflux driven by Na+ gradient was unaffected by TRPM7 silencing. These results suggest that TRPM7 regulates the rate of Mg2+ influx in H9c2 cells, although cytoplasmic Mg2+ homeostasis at basal conditions is unaffected by TRPM7 silencing.
ArticleNumber 47
Audience Academic
Author Inoue, Hana
Konishi, Masato
Yokoyama, Utako
Kobayashi, Ryo
Tashiro, Michiko
Author_xml – sequence: 1
  fullname: Tashiro, Michiko
– sequence: 2
  fullname: Konishi, Masato
– sequence: 3
  fullname: Kobayashi, Ryo
– sequence: 4
  fullname: Inoue, Hana
– sequence: 5
  fullname: Yokoyama, Utako
BookMark eNptUltrFjEQDVKxF_0DPi34IujWZHLdJylF20KLIvU55MtO1pTdpG52i-2vN-1XCkrJwwRyzpk5k7NPdlJOSMhbRg8ZM-pTYSC1ainQllKtob17QfaYMbRVUsHO012oXbJfyhWlQnVgXpFdzikYZuQeObn88f1CNyWOmHxMQ-OWBdPqFizNxQAfmpjCuP6ppfFu7qPzzXSbN6MrS_nYnHYeGo_jWF6Tl8GNBd881gPy8-uXy-PT9vzbydnx0XnrhVRLG0IAYYQ3jvWdQcMYB6UEVd0GKXWgKOtN57k0KLgOilEXggRulAmAIPkB-bzVvV43E_Ye0zK70V7PcXLzrc0u2n9fUvxlh3xjGdVMM66qwvtHhTn_XrEsdorl3oNLmNdiQYiuzqG0qNB3_0Gv8jqn6s-CFJpKMKKrqHaLGtyItu4r18Z-wIS1f_2xUHdrj7SSvJI4VPzhM_h6epyif5YAW4Kfcykzhie7jNr7JNhtEmxNgn1Igr3jfwE8uaPa
Cites_doi 10.1113/jphysiol.2004.067637
10.1161/CIRCULATIONAHA.112.000768
10.1016/j.freeradbiomed.2014.04.015
10.1016/j.bpj.2014.09.015
10.1016/S0006-3495(97)78360-7
10.1073/pnas.1311865110
10.1085/jgp.20028740
10.1016/S0735-1097(97)00524-X
10.1186/1423-0127-19-75
10.1016/j.bbrc.2019.03.178
10.1007/s12576-018-0625-5
10.1073/pnas.1120033109
10.1152/physrev.00012.2014
10.1007/978-3-642-54215-2_21
10.1152/ajpcell.2000.279.6.C1955
10.1016/S0092-8674(03)00556-7
10.1007/s00395-012-0282-4
10.1186/1471-2164-6-48
10.1371/journal.pone.0073171
10.1126/science.1163493
10.1016/j.febslet.2011.05.052
10.1074/jbc.M117.781708
10.1007/s004240000499
10.1529/biophysj.104.055517
10.1152/ajpcell.00074.2006
ContentType Journal Article
Copyright COPYRIGHT 2020 Springer
2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
The Author(s) 2020
Copyright_xml – notice: COPYRIGHT 2020 Springer
– notice: 2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
– notice: The Author(s) 2020
DBID AAYXX
CITATION
3V.
7X7
7XB
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
LK8
M0S
M7P
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
DOI 10.1186/s12576-020-00772-z
DatabaseName CrossRef
ProQuest Central (Corporate)
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
Biological Sciences
Health & Medical Collection (Alumni Edition)
Biological Science Database
Publicly Available Content Database (Proquest) (PQ_SDU_P3)
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle CrossRef
Publicly Available Content Database
ProQuest Central Student
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Central
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList

CrossRef
Publicly Available Content Database
MEDLINE - Academic

Database_xml – sequence: 1
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
EISSN 1880-6562
EndPage 47
ExternalDocumentID A765325432
10_1186_s12576_020_00772_z
GeographicLocations United States
United States--US
GeographicLocations_xml – name: United States
– name: United States--US
GroupedDBID ---
-56
-5G
-BR
-EM
-~C
06C
06D
0R~
0VY
123
199
1N0
203
29L
29~
2JY
2KG
2LR
30V
4.4
408
40D
53G
67N
67Z
6NX
7X7
8FI
8FJ
8TC
95.
96X
AABHQ
AAFWJ
AAIAL
AAJKR
AAJSJ
AARTL
AATVU
AAWCG
AAYIU
AAYQN
AAYTO
AAYXX
AAZMS
ABFTV
ABHLI
ABJOX
ABKCH
ABMNI
ABPLI
ABQBU
ABTEG
ABTHY
ABTMW
ABUWG
ABXPI
ACGFS
ACHXU
ACKNC
ACMLO
ACPRK
ACRMQ
ADBBV
ADHHG
ADHIR
ADINQ
ADKPE
ADUKV
ADURQ
ADZKW
AEGAL
AEGNC
AEJHL
AEJRE
AENEX
AEOHA
AEPYU
AETCA
AEXYK
AFBBN
AFKRA
AFPKN
AFWTZ
AFZKB
AGAYW
AGDGC
AGJBK
AGQMX
AGWZB
AGYKE
AHAVH
AHBYD
AHYZX
AIIXL
AITGF
AJRNO
AKMHD
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AMKLP
AMYQR
ANMIH
ARMRJ
ASPBG
AVWKF
AXYYD
AZFZN
BA0
BAPOH
BBNVY
BENPR
BFQNJ
BHPHI
BMC
C24
C6C
CCPQU
CITATION
CS3
CSCUP
DU5
EBD
EBLON
EBS
EIOEI
EMOBN
ESBYG
F5P
FFXSO
FIGPU
FNLPD
FRRFC
FYUFA
G-Y
G-Z
GGCAI
GGRSB
GJIRD
GQ6
GQ7
GROUPED_DOAJ
HCIFZ
HF~
HG6
HLICF
HMCUK
HMJXF
HRMNR
HZ~
IAO
IBB
IGS
IOF
ITC
ITM
IXC
I~X
J-C
J0Z
JBSCW
JSH
KOV
KPH
M7P
MA-
NQJWS
O93
O9I
O9J
OK1
PIMPY
QOR
QOS
R89
R9I
RJT
ROL
RPM
RSV
S16
S27
S3A
S3B
SBL
SDH
SHX
SISQX
SNE
SNX
SOJ
SPISZ
SSXJD
STPWE
SV3
SZN
T13
TSG
TUS
U2A
U9L
UG4
UKHRP
UZXMN
VC2
VFIZW
W48
WK8
Z45
ZMTXR
ZOVNA
~A9
3V.
7XB
8FE
8FH
8FK
AZQEC
DWQXO
GNUQQ
K9.
LK8
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
ID FETCH-LOGICAL-c456t-fff2484c8a1d98e81132664069be00a2601d89c358e437f610aff523868f2e253
IEDL.DBID RPM
ISSN 1880-6546
IngestDate Tue Sep 17 21:29:19 EDT 2024
Fri Oct 25 08:50:31 EDT 2024
Thu Oct 10 15:49:41 EDT 2024
Wed Nov 13 05:15:56 EST 2024
Wed Nov 13 00:19:19 EST 2024
Thu Nov 21 23:34:50 EST 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Language English
License Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c456t-fff2484c8a1d98e81132664069be00a2601d89c358e437f610aff523868f2e253
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0002-2680-6702
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10717136/
PMID 33028185
PQID 2547052849
PQPubID 2029107
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_10717136
proquest_miscellaneous_2449260674
proquest_journals_2547052849
gale_infotracgeneralonefile_A765325432
gale_infotracacademiconefile_A765325432
crossref_primary_10_1186_s12576_020_00772_z
PublicationCentury 2000
PublicationDate 2020-10-07
PublicationDateYYYYMMDD 2020-10-07
PublicationDate_xml – month: 10
  year: 2020
  text: 2020-10-07
  day: 07
PublicationDecade 2020
PublicationPlace Heidelberg
PublicationPlace_xml – name: Heidelberg
– name: London
PublicationTitle The journal of physiological sciences
PublicationYear 2020
Publisher Springer
BioMed Central
Publisher_xml – name: Springer
– name: BioMed Central
References M Tashiro (772_CR9) 1997; 73
R Macianskiene (772_CR3) 2012; 19
H Inoue (772_CR12) 2014; 72
YH Zhang (772_CR16) 2012; 107
J Jin (772_CR24) 2008; 322
R Sah (772_CR17) 2013; 110
J Jin (772_CR25) 2012; 109
S Castiglioni (772_CR26) 2019; 513
A Goytain (772_CR5) 2005; 6
M Tashiro (772_CR8) 2019; 69
M Watanabe (772_CR10) 2001; 442
A Gwanyanya (772_CR14) 2004; 559
M Tashiro (772_CR6) 2014; 107
A Gwanyanya (772_CR15) 2006; 291
R Sah (772_CR18) 2013; 128
M Tashiro (772_CR2) 2013; 8
M Tashiro (772_CR21) 2000; 279
MK Monteilh-Zoller (772_CR4) 2003; 121
MC Haigney (772_CR28) 1998; 31
H Nguyen (772_CR19) 2014; 2
A Fleig (772_CR13) 2014; 222
M Watanabe (772_CR27) 2004; 9
M Tashiro (772_CR7) 2019; 69
JH de Baaij (772_CR1) 2015; 95
F Deason-Towne (772_CR23) 2011; 585
C Schmitz (772_CR22) 2003; 114
P Tursun (772_CR11) 2005; 88
B Reilly-O'Donnell (772_CR20) 2017; 292
References_xml – volume: 559
  start-page: 761
  year: 2004
  ident: 772_CR14
  publication-title: J Physiol
  doi: 10.1113/jphysiol.2004.067637
  contributor:
    fullname: A Gwanyanya
– volume: 128
  start-page: 101
  year: 2013
  ident: 772_CR18
  publication-title: Circulation
  doi: 10.1161/CIRCULATIONAHA.112.000768
  contributor:
    fullname: R Sah
– volume: 72
  start-page: 257
  year: 2014
  ident: 772_CR12
  publication-title: Free Radic Biol Med
  doi: 10.1016/j.freeradbiomed.2014.04.015
  contributor:
    fullname: H Inoue
– volume: 9
  start-page: 181
  year: 2004
  ident: 772_CR27
  publication-title: Exp Clin Cardiol
  contributor:
    fullname: M Watanabe
– volume: 107
  start-page: 2049
  year: 2014
  ident: 772_CR6
  publication-title: Biophys J
  doi: 10.1016/j.bpj.2014.09.015
  contributor:
    fullname: M Tashiro
– volume: 73
  start-page: 3358
  year: 1997
  ident: 772_CR9
  publication-title: Biophys J
  doi: 10.1016/S0006-3495(97)78360-7
  contributor:
    fullname: M Tashiro
– volume: 110
  start-page: E3037
  year: 2013
  ident: 772_CR17
  publication-title: Proc Natl Acad Sci USA
  doi: 10.1073/pnas.1311865110
  contributor:
    fullname: R Sah
– volume: 121
  start-page: 49
  year: 2003
  ident: 772_CR4
  publication-title: J Gen Physiol
  doi: 10.1085/jgp.20028740
  contributor:
    fullname: MK Monteilh-Zoller
– volume: 31
  start-page: 701
  year: 1998
  ident: 772_CR28
  publication-title: J Am Coll Cardiol
  doi: 10.1016/S0735-1097(97)00524-X
  contributor:
    fullname: MC Haigney
– volume: 19
  start-page: 75
  year: 2012
  ident: 772_CR3
  publication-title: J Biomed Sci
  doi: 10.1186/1423-0127-19-75
  contributor:
    fullname: R Macianskiene
– volume: 513
  start-page: 159
  year: 2019
  ident: 772_CR26
  publication-title: Biochem Biophys Res Commun
  doi: 10.1016/j.bbrc.2019.03.178
  contributor:
    fullname: S Castiglioni
– volume: 69
  start-page: 97
  year: 2019
  ident: 772_CR7
  publication-title: J Physiol Sci
  doi: 10.1007/s12576-018-0625-5
  contributor:
    fullname: M Tashiro
– volume: 109
  start-page: E225
  year: 2012
  ident: 772_CR25
  publication-title: Proc Natl Acad Sci USA
  doi: 10.1073/pnas.1120033109
  contributor:
    fullname: J Jin
– volume: 95
  start-page: 1
  year: 2015
  ident: 772_CR1
  publication-title: Physiol Rev
  doi: 10.1152/physrev.00012.2014
  contributor:
    fullname: JH de Baaij
– volume: 222
  start-page: 521
  year: 2014
  ident: 772_CR13
  publication-title: Handb Exp Pharmacol
  doi: 10.1007/978-3-642-54215-2_21
  contributor:
    fullname: A Fleig
– volume: 279
  start-page: C1955
  year: 2000
  ident: 772_CR21
  publication-title: Am J Physiol Cell Physiol
  doi: 10.1152/ajpcell.2000.279.6.C1955
  contributor:
    fullname: M Tashiro
– volume: 114
  start-page: 191
  year: 2003
  ident: 772_CR22
  publication-title: Cell
  doi: 10.1016/S0092-8674(03)00556-7
  contributor:
    fullname: C Schmitz
– volume: 107
  start-page: 282
  year: 2012
  ident: 772_CR16
  publication-title: Basic Res Cardiol
  doi: 10.1007/s00395-012-0282-4
  contributor:
    fullname: YH Zhang
– volume: 6
  start-page: 48
  year: 2005
  ident: 772_CR5
  publication-title: BMC Genom
  doi: 10.1186/1471-2164-6-48
  contributor:
    fullname: A Goytain
– volume: 8
  start-page: e73171
  year: 2013
  ident: 772_CR2
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0073171
  contributor:
    fullname: M Tashiro
– volume: 322
  start-page: 756
  year: 2008
  ident: 772_CR24
  publication-title: Science
  doi: 10.1126/science.1163493
  contributor:
    fullname: J Jin
– volume: 585
  start-page: 2275
  year: 2011
  ident: 772_CR23
  publication-title: FEBS Lett
  doi: 10.1016/j.febslet.2011.05.052
  contributor:
    fullname: F Deason-Towne
– volume: 69
  start-page: S169
  year: 2019
  ident: 772_CR8
  publication-title: J Physiol Sci
  doi: 10.1007/s12576-018-0625-5
  contributor:
    fullname: M Tashiro
– volume: 292
  start-page: 13361
  year: 2017
  ident: 772_CR20
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M117.781708
  contributor:
    fullname: B Reilly-O'Donnell
– volume: 442
  start-page: 35
  year: 2001
  ident: 772_CR10
  publication-title: Pflugers Arch
  doi: 10.1007/s004240000499
  contributor:
    fullname: M Watanabe
– volume: 2
  start-page: 179
  year: 2014
  ident: 772_CR19
  publication-title: J Cardiovasc Dis Diagn
  contributor:
    fullname: H Nguyen
– volume: 88
  start-page: 1911
  year: 2005
  ident: 772_CR11
  publication-title: Biophys J
  doi: 10.1529/biophysj.104.055517
  contributor:
    fullname: P Tursun
– volume: 291
  start-page: C627
  year: 2006
  ident: 772_CR15
  publication-title: Am J Physiol Cell Physiol
  doi: 10.1152/ajpcell.00074.2006
  contributor:
    fullname: A Gwanyanya
SSID ssj0046928
Score 2.2667854
Snippet TRPM7, a member of the melastatin subfamily of transient receptor potential channels, is suggested to be a potential candidate for a physiological Mg 2+...
TRPM7, a member of the melastatin subfamily of transient receptor potential channels, is suggested to be a potential candidate for a physiological Mg.sup.2+...
TRPM7, a member of the melastatin subfamily of transient receptor potential channels, is suggested to be a potential candidate for a physiological Mg2+...
SourceID pubmedcentral
proquest
gale
crossref
SourceType Open Access Repository
Aggregation Database
StartPage 1
SubjectTerms Antibodies
Calcium
Efficiency
Ethylenediaminetetraacetic acid
Fluorescent indicators
Homeostasis
Magnesium
Myoblasts
Physiology
Scientific equipment and supplies industry
Short Communication
Transient receptor potential proteins
SummonAdditionalLinks – databaseName: Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3da9swED-27mUvox8bc9sNFUb30InakizLTyWMtWGQMUYLeRO2LHV5iNPWCbT963enONlSyp78IIHw3em-dPc7gE8yV03pGuQALnO0147XZZ1yerOpZCFDGqh3ePRDD6_U93E-7hNuXV9WudKJUVE3M0c58lMMZIo0R2Vant3ccpoaRa-r_QiNl_AqE2jKUZ6L8TrgwsgvzlYlyDFOTTurphmjT7uMPG1OwRMh2gj-uGGYnqrnpyWT_9ig82140zuPbLDk9g688O0u7A1aDJynD-yYxXLOmCffg4vLXz9HBesm1FWE9okRkGa7INeSja7FCZvQeJJ7_DAXpcSx6cOsRmd63n1hw9IJRkn97i1cnX-7_Drk_dQE7tAZmvMQglBGOVNlTWm8oVHyWlODa-3TtCIIscaUTubGK1kEdJ-qEDAcNdoE4UUu38FWO2v9e2BCh0w2XqmqblSqfK117lVapbrWLvc-gZMVyezNEhzDxqDCaLsksEUC20hg-5jAZ6KqpZuDNHRV3wCAZxEGlR0UOpfUmy8SON7Yeb1E4H5u4-GKQ7a_e539KykJHK2X8dYQ1arWzxa4RxFQIlpqlYDZ4Oz6Rwh3e3OlnfyO-NsZxcCZ1Pv_P_0AXguSL6o2KA5ha3638B_Qg5nXH6OY_gEKGe2A
  priority: 102
  providerName: ProQuest
Title TRPM7 silencing attenuates Mg2+ influx in cardiac myoblasts, H9c2 cells
URI https://www.proquest.com/docview/2547052849
https://search.proquest.com/docview/2449260674
https://pubmed.ncbi.nlm.nih.gov/PMC10717136
Volume 70
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Ra9swED7aDkZfxrpuzFsXNBjdQ6fGtmRZfsxK2zBIKaOFvAlblrrA4pQ5gbW_fndyXJbSpz754WRsn066--S77wC-iEzWha1xBlDM0V9bXhVVzOmfTSly4WNPtcOTCzW-lj-m2XQLVF8LE5L2bTU7bn7Pj5vZr5BbeTu3wz5PbHg5OUkIhCRCDbdhG_1vj9G7_RfxXuioSkRjnEp1-lIZrYZtQvE1J8hEPDYpv9-Fl4joAyXShmd6vD8_zpn8zwmdvYZX6-iRjbq33IMt17yB_VGDyHl-xw5ZyOcMB-X7cH6Fr5uzdkZlReigGDFpNiuKLdnkJj1iM-pP8hcvzAYzsWx-t6gwml6239i4sCmjU_32LVyfnV6djPm6bQK3GA0tufc-lVpaXSZ1oZ2mXvJKUYVr5eK4JA6xWhdWZNpJkXuMn0rvEY9qpX3q0ky8g51m0bj3wFLlE1E7KcuqlrF0lVKZk3EZq0rZzLkIjnqVmduOHcMEVKGV6XRtUNcm6NrcR_CVtGpo6aAObbmuAMBnEQmVGeUqE1Scn0ZwuDHypqPgfmrgQT9DZr34WoOSPM7Q7xYRfH4Q47IhrZWNW6xwjCSmRHTVMgK9MbMPH0LE25sStMdAwN3b34fn3_oRdlMyQ0pFyA9gZ_ln5T5heLOsBmjT03wAL76fXlz-HIRDgkGw8H_CwfpA
link.rule.ids 230,314,727,780,784,864,885,12056,21388,27924,27925,31719,31720,33744,33745,43310,43805,53791,53793,73745,74302
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB7B9gAXVCiIQClGQuVQrCbxI86pWlDLAt1VVW2l3qzEscsemi1kV2r765nJJoWtEKccbMnKzHhenvkG4L1QsspdhRzAZY722vEyL2NObzaFyESIA_UOjyd6dCa_navzLuHWdGWVvU5sFXU1d5Qj38dAJosVKtP84Oonp6lR9LrajdB4CBuEnK4GsPHpcHJy2utijP3a6aoEOsapbadvmzF6v0nI1-YUPhGmTcpv10zTfQV9v2jyLyt0tAlPOveRDVf8fgoPfP0MtoY1hs6XN2yXtQWdbaZ8C75MT0_GGWtm1FeEFooRlGa9JOeSjS_SPTajASXX-GGulRPHLm_mJbrTi-YjG-UuZZTWb57D2dHh9POId3MTuEN3aMFDCKk00pkiqXLjDQ2T15paXEsfxwWBiFUmd0IZL0UW0IEqQsCA1GgTUp8q8QIG9bz2L4GlOiSi8lIWZSVj6UutlZdxEetSO-V9BHs9yezVCh7DtmGF0XZFYIsEti2B7W0EH4iqlu4O0tAVXQsAnkUoVHaYaSWoOz-NYHdt58UKg_tfG7d7Dtnu9jX2j6xE8O5uGe8NUa2o_XyJeyRBJaKtlhGYNc7e_Qghb6-v1LMfLQJ3QlFwIvSr_5_-Fh6NpuNje_x18v01PE5J1qj2INuGweLX0r9Bf2ZR7nRC-xtxJ_HW
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3db9MwED9BJyFeEDAQgQFGQuNhWE0cx3GeUIGV8tGqmjZpb1bi2KMPSwdpJba_nrvUGXRCPOXBlqzc99l3vwN4nWayLmyNHMBljv7a8qqoYk5vNmWapz721Ds8nanJifxymp2G-qc2lFX2NrEz1PXS0h35EBOZPM7QmBZDH8oi5h_H7y5-cJogRS-tYZzGbdhBrxiLAey8P5zNj3q7jHlgN2mVAMg4tfD0LTRaDduE4m5OqRTh2wh-teWmbhrrmwWUf3mk8X24F0JJNtrw_gHccs1D2B01mEafX7J91hV3drfmu_Dp-Gg-zVm7oB4j9FaMYDWbNQWabHomDtiChpX8wg-zncxYdn65rDC0XrVv2aSwgtEVf_sITsaHxx8mPMxQ4BZDoxX33guppdVlUhfaaRosrxS1u1YujksCFKt1YdNMO5nmHoOp0ntMTrXSXjiRpY9h0Cwb9wSYUD5JaydlWdUylq5SKnMyLmNVKZs5F8FBTzJzsYHKMF2KoZXZENgggU1HYHMVwRuiqiE9QhraMrQD4FmESGVGucpS6tQXEexv7Tzb4HH_a-NezyETNLE1f-QmglfXy6hDRLWyccs17pEEm4h-W0agtzh7_SOEwr290iy-d2jcCWXESaqe_v_0l3AH5dV8-zz7-gzuChI1KkPI92Cw-rl2zzG0WVUvgsz-BsbG9gM
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=TRPM7+silencing+attenuates+Mg2%2B+influx+in+cardiac+myoblasts%2C+H9c2+cells&rft.jtitle=The+journal+of+physiological+sciences&rft.au=Tashiro%2C+Michiko&rft.au=Konishi%2C+Masato&rft.au=Kobayashi%2C+Ryo&rft.au=Inoue%2C+Hana&rft.date=2020-10-07&rft.pub=Springer&rft.issn=1880-6546&rft.volume=70&rft.issue=1&rft_id=info:doi/10.1186%2Fs12576-020-00772-z&rft.externalDocID=A765325432
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1880-6546&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1880-6546&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1880-6546&client=summon