Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

• Published in: Genes & development Vol. 33; no. 23-24; pp. 1619 - 1634
• Main Authors: McSwiggen, David T., Mir, Mustafa, Darzacq, Xavier, Tjian, Robert
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.12.2019
• Subjects: Cell Biology - trends; Cell Physiological Phenomena - physiology; Cytological Techniques - standards; Fluorescence Recovery After Photobleaching - standards; Gene Expression Regulation - physiology; Liquid-Liquid Extraction; Transcription Factors - metabolism; phase separation; condensate; liquid–liquid phase separation; fluorescence recovery after photobleaching

The idea that liquid–liquid phase separation (LLPS) may be a general mechanism by which molecules in the complex cellular milieu may self-organize has generated much excitement and fervor in the cell biology community. While this concept is not new, its rise to preeminence has resulted in renewed in...