A fungal anticodon nuclease ribotoxin exploits a secondary cleavage site to evade tRNA repair

• Published in: RNA (Cambridge) Vol. 18; no. 9; pp. 1716 - 1724
• Main Authors: Meineke, Birthe, Kast, Alene, Schwer, Beate, Meinhardt, Friedhelm, Shuman, Stewart, Klassen, Roland
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.09.2012
• Subjects: Amino Acid Sequence; Histone Acetyltransferases - genetics; Histone Acetyltransferases - metabolism; Killer Factors, Yeast - chemistry; Killer Factors, Yeast - genetics; Killer Factors, Yeast - metabolism; Models, Biological; Molecular Sequence Data; Mutation; Phylogeny; Ribonucleases - chemistry; Ribonucleases - genetics; Ribonucleases - metabolism; RNA, Transfer - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Sequence Alignment

PaOrf2 and γ-toxin subunits of Pichia acaciae toxin (PaT) and Kluyveromyces lactis zymocin are tRNA anticodon nucleases. These secreted ribotoxins are assimilated by Saccharomyces cerevisiae, wherein they arrest growth by depleting specific tRNAs. Toxicity can be recapitulated by induced intracellul...